Indian Journal of Pharmaceutical Sciences
Users online: 48
Scientific Publication of the Indian Pharmaceutical Association
Home Email this page Print this page Bookmark this page Decrease font size Default font size Increase font size
The Journal Search Current Issue Archives Instructions Online submission Login  


 
SHORT COMMUNICATION
Year : 2008  |  Volume : 70  |  Issue : 2  |  Page : 249-251
Spectrophotometeric determination of cefuroxime axetil from bulk and in its tablet dosage form


Department of Pharmaceutical Chemistry, Bharati Vidyapeeth College of Pharmacy and Research Center, Near Chitranagari, Kolhapur-416 013, India

Date of Submission08-Mar-2006
Date of Decision22-Oct-2007
Date of Acceptance21-Apr-2008

Correspondence Address:
M V Shinde
Department of Pharmaceutical Chemistry, Bharati Vidyapeeth College of Pharmacy and Research Center, Near Chitranagari, Kolhapur-416 013
India
Login to access the Email id


DOI: 10.4103/0250-474X.41468

PMID: 20046725

Get Permissions

   Abstract 

A simple rapid spectrophotometric method has been developed for estimation of cefuroxime axetil from bulk drug and tablet dosage form by using 1-nitroso-2-napthol and sodium hydroxide. The method is based on the formation of yellow-orange coloured complex of drug with 1-nitroso-2-napthol having absorbance maxima at 424 nm. The Beer's law is obeyed in the concentration range of 10-50 μg/ml of the drug but more precisely it obeys in the range of 10- 30 mg/ml. The slope and intercept values are 0.0101 and 0.0838, respectively. Results of analysis of this method were validated statistically and by recovery studies. The method is applied to the marketed tablet formulation. Result of analysis of tablet formulation given as percentage of label claim ±standard deviation is 99.17±1.57. The precision and accuracy was examined by performing recovery studies and was found to be 99.50±1.82. Sandell's correlation coefficient is calculated as 0.4434. The developed method is simple, sensitive and reproducible and can be used for routine analysis of cefuroxime axetil from bulk and tablet dosage form.


Keywords: Cefuroxime axetil, yellow orange complex, spectrophotometry,1-nitroso-2-napthol


How to cite this article:
Shinde M V, Pishawikar S A, More H N. Spectrophotometeric determination of cefuroxime axetil from bulk and in its tablet dosage form. Indian J Pharm Sci 2008;70:249-51

How to cite this URL:
Shinde M V, Pishawikar S A, More H N. Spectrophotometeric determination of cefuroxime axetil from bulk and in its tablet dosage form. Indian J Pharm Sci [serial online] 2008 [cited 2014 Aug 21];70:249-51. Available from: http://www.ijpsonline.com/text.asp?2008/70/2/249/41468


Cefuroxime is chemically (6R,7R)-3-carbamoy- loxymethyl-7-[(Z)-2-(2-furyl)-2-(methoxyimino)acetamido]-ceph-3-em-4-carboxylic acid. Cefuroxime is official in Indian pharmacopoeia. It is the first of the series of alpha methoxyiminoacyl substituted cephalosporins that constitute most of the third generation agents available for clinical use. It is active against some beta lactamase strains that are resistant to cefamandole. The literature survey revealed that various methods of analysis for cefuroxime alone or in combination with other drugs have been reported, which included, HPLC [1],[2],[3],[4] , electrokinetic [5] , HPTLC [6] and spectrophotometric methods [7] .

The method developed is based on the formation of diazo complex of cefuroxime axetil with 1-nitroso-2-napthol in the presence of sodium hydroxide to give yellow orange coloured chromogen with 'λmax 424 nm. Reaction conditions were optimized to obtain maximum colour intensity. The method is simple, reproducible and requires low cost and method is applied successfully to the analysis of the marketed tablet formulation.

A Double beam JascoV-530 Model spectrophotometer having 2 matched cells with 1-cm light path was employed for spectral measurements. The tablet dosage form was procured from local market. Sodium hydroxide (0.006 N) was prepared by alligation method. 1-nitroso-2-napthol (25 mg) was weighed accurately and dissolved in 25 ml with double distilled water. Cefuroxime axetil (5 mg) was weighed accurately and dissolved in double distilled water to produce a 100 μg/ml solution.

Working standard solution containing 100 μg/ml of cefuroxime axetil was prepared in double distilled water. Cefuroxime axetil was treated with 1-nitroso-2-napthol and sodium hydroxide leading to formation of yellow- orange coloured complex. The analyte gave maximum absorbance at 424 nm. In this method volume of 1-nitroso-2-napthol (concentration 0.001 mg/ml) was optimized to 2.5 ml.

Using various normality ranges from 1 N to 0.006 N the normality of sodium hydroxide was optimized. Sodium hydroxide of normality equivalent to 0.006 N was found to yield reproducible results. Beer's law is obeyed in concentration range of 10 to 30 μg/ml. The slope and intercept values are 0.0101 and 0.0838. The correlation coefficient was found to be 0.9990. To study the recovery of cefuroxime axetil, drug from the tablet sample was taken to which different quantities of pure drug (reference standard) was added within the analytical concentration range in the proposed method. The added quantity of individual drug was estimated in the method. % concentration ±SD and coefficient of variance for cefuroxime axetil bulk drug (AS) and cefuroxime axetil recovery sample were found to be 100.27± 1.63,99.50±1.82 and 1.138, 1.1134, respectively. From these values it seems that method is accurate and reproducible for both bulk drug and formulation.

The film coated marketed tablet formulation with 500 mg of drug claim are used for applying developed method on formulation. Twenty tablets of marketed drug were weighed and powdered. The powder equivalent to 5 mg of cefuroxime axetil was weighed accurately and treated with double distilled water (50 ml) to produce 100 μg/ml of the drug solution. The mixture was sonicated for 15 min and filtered through Whatmann filter paper No. 40. The dilutions were made accordingly to concentration range in the given procedure. % concentration ±SD and coefficient of variance for the tablet formulation was found to be 99.17±1.57 and 1.139, respectively.

In this method cefuroxime axetil reacts with 1-nitroso-2-napthol and sodium hydroxide to give yellow orange coloured complex with stability up to 2 h. The complex exhibit maximum absorbance at 424 nm. The Beer's law was obeyed in the concentration range of 10 to 30 μg.

These reaction conditions were optimized to obtain maximum colour intensity. Proposed method was use for pure bulk drug as well as for marketed formulation. The result obtained compared favorably with labeled amount of drug as well as that of the formulation. None of the usual diluent, lubricant, film formers employed in preparation of tablet dosage form was found to interfere in the proposed procedure. The proposed method hence is specific, precise, accurate and reliable.


   Acknowledgements Top


Sincere thanks are due to M/s Glenmark Labs Ltd, for providing gift sample of drug and Dr. H. N. More, Principal of Bharati Vidyapeeth College of Pharmacy, Kolhapur for providing necessary facilities for experimentation.

 
   References Top

1.Zivanovic L, Vladimirov S, Zeceric M. Invesigations of chromatographic conditions for the separation of cefuroxime axetil and its geometric isomer. J Chromatogr Analyst Technol Biomed Life Sci 2004; 5:175-77.  Back to cited text no. 1    
2.Zajac M, Dobrowolski L, Osczaprwicz I. Stability testing of cefuroxime in tablets by micellar liquid chromatography. J Pharm Biomed Anal 2003; 32:1181-83.  Back to cited text no. 2    
3.Peleman R, Hoorekebe H, Pauwels R. Measurement of cefuroxime in human alveolar lavage fluid by high performance liquid chromatography after solid phase extraction. J Chromatogr B Biomed Sci Appl 1997; 689: 438-41.   Back to cited text no. 3    
4. Altria KD, Campbell CJ, Rogan MM. Reduction in sample pretreatment required by using high performance capillary electrokinetic separation methods. J Pharm Biomed Anal 1990; 8:1005-07.   Back to cited text no. 4    
5. Sciacchitano CJ, Mopper B, Specchio J. Identification and separation of five cephalosporins by micellar electrokinetic capillary chromatography. J Chromatogr B Biomed Appl 1994; 657:395.  Back to cited text no. 5    
6.Dhaneshwar SR, Kadam SS, Sirisha DV. Development and validation of a HPTLC method for simultaneous estimation of cefuroxime axetil and probenecid. Indian J Pharm Sci 2004; 66:278-80.  Back to cited text no. 6    
7.Pistos C, Kalovidouris M, Kantopoulos G. Application of the ion pair concept to the n-octanol-water partitioning of cefepime and cefpirome. Int J Clin Pharmacol Ther 2004; 42:367-69.   Back to cited text no. 7    




 

Top
Print this article  Email this article
 
  Search
   
   Next article
   Previous article 
   Table of Contents
  
    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
    Article in PDF (102 KB)
    Citation Manager
    Access Statistics
    Reader Comments
    Email Alert *
    Add to My List *
* Registration required (free)  


    Abstract
    Acknowledgements
    References

 Article Access Statistics
    Viewed2957    
    Printed54    
    Emailed4    
    PDF Downloaded372    
    Comments [Add]    

Recommend this journal