- *Corresponding Author:
- D. Lohithasu
Division of Pharmaceutical Technology, Andhra University College of Pharmaceutical Sciences, Visakhapatnam-530 003, India
E-mail: [email protected]
|Date of Submission||01 Sep 2015|
|Date of Revision||29 Feb 2016|
|Date of Acceptance||09 Apr 2016|
|Indian J Pharm Sci, 2016; 78(2):224-230|
The objective of this present study was to investigate binder property of Lannea coromandelica in tablet formulations and its comparative binding property with povidone as standard binder. All the eight tablet batches (F-1 to F-8) were prepared by wet granulation technique. Microcrystalline cellulose (Prosolv SM) as diluent, diclofenac sodium as model drug using 4, 6, 8 and 10% of L. coromandelica gum used as a novel binder, magnesium stearate used as an antiadherent and talc used as lubricant. Micromeritic properties of granules such as bulk density, tapped density, compressibility index, hausner’s ratio, angle of repose as well as physical parameters of the compressed tablets like hardness, friability, thickness and disintegration time were determined and found to be satisfactory. The Fourier transform infrared spectroscopic analysis showed that the tablet formulation containing L. coromandelica gum is compatible with the drug and other excipients, which are used in the tablet formulation. Thus, concluded that L. coromandelica gum has role as a potential binder in tablets formulations. The in vitro drug release profile concluded that tablets formulations containing L. coromandelica gum 20 mg/200 mg of total weight of tablet as binder showed better results as compared to povidone as standard binder.
Lannea coromandelica, plant gum, binding ability, plant natural polymer, Diclofenac sodium and FTIR studies
In the present day, polymers those are derived from plant origin play a vital role as pharmaceutical applications such as filler, binder, disintegrant in tablets, protective colloids in suspensions, thickeners in oral liquids, gelling agents in gels and bases in suppository . Natural gums are obtained as an outcome of metabolic mechanism of plants from natural sources either absorbs water to form a viscous solution or water soluble . Plant derived natural polysaccharides are more suitable as pharmaceutical excipients due to nontoxicity, availability, stability, renewability and are, therefore, extensively used as a matrix forming agent in modified release dosage forms .
Natural gums are also useful in formulating immediate and sustained release preparations [4-6]. The natural excipients are preferred to synthetic and semi synthetic ones because of their lack of toxicity, soothing action, low cost, availability and non-irritant nature of the excipients also they are biocompatible, less expensive, and easily available than the synthetic ones  .
The isolated gum from the tree Lannea coromandelica (gumpena) has been established to be a neutral polysaccharide composed of d-galactose and l-arabinose in the 4:1 ratio . Natural polysaccharide gumpena gum (GG) obtained from the plant of L. coromandelica (Houtt.) Merr. (Anacardiaceae). Upon injury of epithelial cells, the bark of the mature (6 to 10 year old) L. coromandelica (Houtt.) Merr. tree produces a gum that is exuded at the point of injury. The gum also exudes naturally . Gumpena gum tree is abundantly found in forests of Srikakulam, Visakhapatnam and West Godavari districts in Andhra Pradesh, Rawatbhata, near Kota, Rajasthan, India.
Earlier pharmacological studies on L. coromandelica reported antiinflammatory , antimicrobial [11,12], hypotensive , wound healing and aphrodisiac activities. The plant also illustrated its beneficial effect on ulcerative stomatitis, dyspepsia, general debility, gout, cholera, diarrhoea, dysentery , sore eyes, leprosy, sprains and bruises , elephantiasis , eruptions, snakebite, stomach ache, and vaginal trouble [17,18]. The plant gum is given in sprains , asthma and as a cordial to women during lactation, bark extract have antimicrobial and antifungal activity  .
L. coromandelica is a medium-sized deciduous tree belonging to family Anacardiaceae. They are useful for imparting the structural strength required during the processing, handling and packaging of tablets as well as these plant gums are of non-toxic nature and widely available . No work has been done on L. coromandelica gum as tablet binder. Hence the gum is widely available and need to be evaluated by using as an excipient in tablet formulations.
Materials and Methods
Diclofenac sodium is obtained as a gift sample from Crips Laboratory Pvt. Ltd, Visakhapatnam. Microcrystalline cellulose (Prosolv SM, JRS Pharma, Germany), magnesium stearate, talc (Reachem Lab. Chemicals Pvt. Ltd, Chennai) and povidone was provided by Hetero Pharma, Hyderabad. All other chemicals used were of analytical grade.
Collection and isolation of L. coromandelica gum from crude gum
Gum was collected from the plants of L. coromandelica in May-June, 2014 from Srikakulam and Visakhapatnam districts, India. After collection, gum was dried in oven (M/s. Shiv Scientific Services, India) at 60° and removed extraneous materials from the crude gum. The gum was then precipitated from the solution using ethanol. The precipitate was separated, dried in oven at 50° and ground by high speed mechanical blender (Model no. Butterfly, LCM-2306-135, India). The resultant powder was sifted through mesh no. 100 and stored in a tightly closed container for further use.
Preparation and evaluation of granules
Granules of various formulation batches (F1- F8) containing 50 mg of diclofenac sodium were prepared by wet granulation method. Natural gum (L. coromandelica) in different ratios was used as binder and povidone as a standard binder. Diclofenac sodium, microcrystalline cellulose and gum were separately mixed and required quantity of water (as granulating agent) was added. The obtained damp mass was passed through mesh no. 12. The obtained granules were dried at 60° for 4-5 h, and then passed through mesh no. 18. Finally, magnesium stearate, talc (as antiadherent and lubricant) was added to every batch prior to compression of granules. All the formulation batches are shown in Table 1.
|L. coromandelica gum||8||12||16||20||--||--||--||--|
Table 1: Formulation Of Different Batches (F1 -F8) Of Diclofenac Sodium Tablets
|Bulk density (g/ml)||0.567±0.00||0.582±0.01||0.601±0.01||0.601±0.00||0.487±0.00||0.489±0.00||0.484±0.00||0.603±0.01|
|Tapped density (g/ml)||0.626±0.01||0.652±0.01||0.662±0.01||0.65±0.01||0.583±0.00||0.578±0.00||0.58±0.01||0.664±0.01|
|Angle of repose(°)||32.02||31.41||29.31||29.34||35.86||34.09||33.47||30.32|
*All the values are expressed as mean ±SD
Table 2: Evaluation Of Granules
Evaluation of granules
The bulk density, tapped density, Hausner’s ratio, compressibility index and angle of repose were determined for each batch of dried granules.
Bulk density is the ratio of total mass of granules to the bulk volume of granules. It was measured by pouring the known weighed of granules (m) into 10 ml graduated cylinder, and the unsettled volume (V0) was noted. It is expressed in g/ml. It is calculated by, m/V0 .
Tapped density is the ratio of total mass of granules to the tapped volume of granules. It is determined by placing a graduated cylinder (10 ml) containing known weight (m) of granules, was tapped on a hard surface for fixed number of taps until the powder bed volume has reached a minimum volume. Observed tapped volume (VT) was noted. Tapped density was calculated by, m/ VT .
Carr’s compressibility index was determined the formula,
Carr’s compressibility index was determined using the formula, Carr’s compressibility index = (tapped density-bulk density)/ tapped density.
Hausner’s ratio is the ratio of tapped density and bulk density and was calculated using the formula , Hausner’s ration=tapped density/bulk density.
The accurately weighed granules were taken in a funnel. The funnel height was adjusted in such a way that the tip of the funnel just touched the apex of the heap of the granules. The granules were allowed to flow through the funnel freely on to the surface. The diameter of the cone was measured (funnel method). The angle of repose (θ) values were calculated using the formula, Tan θ = h/r, where h is the height of the heap and r is the radius of the heap.
Preparation of diclofenac tablets
Tablets were prepared by compressing granules on compression machine using concave punch (8 mm). Total weight of tablet was kept as 200 mg (M/s. Cadmach Machinery Co. Pvt. Ltd, India).
Evaluation of tablets
After compression twenty tablets were selected at random, weighed together and individually by analytical balance (AX120, M/s. Shimadzu Corporation, Japan), then average weight and standard deviation was determined  .
From each batch, 10 tablets were randomly collected, accurately weighed and powdered in glass mortar. Powder amount equivalent to 50 mg of diclofenac sodium was shaken with 60 ml of methanol in a 200 ml volumetric flask and final volume was made up to the mark with methanol, from this solution 5 ml was taken, then further diluted to 100 ml with methanol and absorbance was measured using spectrophotometer (UV-Elico Pvt. Ltd., SL 210, India) at 276 nm. The content was determined by preparing same concentration of sodium diclofenac in the same solvent and absorbance was measured at 276 nm. The % drug content was determined by , % drug content = ((Abs sample × average weight of tablet)/(Abs standard × weight of sample))×100.
From each batch, the hardness of 5 tablets was determined by using Monsanto hardness tester and mean±SD of each formulation mentioned in Table 3. From each batch, thickness of 10 tablets was determined using vernier callipers and average values were calculated. The friability of tablets (10) was determined using Roche friabilator (EF-2, M/s. Electrolab, India). Ten tablets were initially weighed (W0) and transferred into friabilator. The friabilator was operated at 25 rpm for 4 minutes or was run up to 100 revolutions. The tablets were dedusted by using camel hair brush and weighed again (W). Percent friability was then calculated using the formula, % Friability= (W0-W)/W0)×100, where W0 is the initial weight of 10 tablets and W is the weight of 10 tablets after 100, revolutions. % Friability of tablets less than 1% were considered acceptable.
|Formulation||Hardness (Kg/cm2)||Weight variation (%)||Thickness (mm)||Friability* (%)||Disintegration time* (min)||Drug content (%)|
|F1||5 - 6||200.60±3.24||4.33±1.03||0.69||3.2||98.05±0.55|
|F2||5 - 6||200.20±3.15||4.31±1.02||0.53||8.1||98.95±0.65|
|F3||5 - 6||200.10±2.58||4.34±0.53||0.16||8.8||100.11±0.58|
|F4||5 - 6||200.67±3.22||4.31±1.10||0.42||14.1||100.14±1.09|
|F5||5 - 6||199.25±3.76||4.36±1.10||0.38||4.4||98.42±0.72|
|F6||5 - 6||202.50±3.27||4.35±1.03||0.28||9.0||100.01±0.96|
|F7||5 - 6||200.38±3.36||4.29±0.81||0.17||14.4||99.26±1.04|
|F8||5 - 6||200.19±2.48||4.30±0.52||0.32||9.4||99.34±0.98|
*All the values are expressed as mean±SD except data denoted by asterisk
Table 3: Physical Characterization Of Diclofenac Sodium Tablets
Disintegration test apparatus (DBK Instrument, England, 40 TDA01) containing 100 ml of 0.1 N HCl as disintegration medium and thermostatically maintained at 37±2°. Six tablets from randomly selected from each batch, placing one per cylindrical tube, were tested at a time. The time taken for each of the six tablets to disintegrate was recorded and the disintegration time of each batch was calculated .
In vitro drug dissolution studies
In vitro dissolution test of the all the batches compressed diclofenac sodium tablet was performed using USP dissolution apparatus 2 (Disso 2000,M/s. Labindia, India), using pH 6.8 phosphate buffer as dissolution medium. The temperature was maintained at 37±2º, at a rotation speed of 50 rpm. The 5 ml of filtered samples were withdrawn at regular intervals up to 60 minutes, then replacing 5 ml of fresh dissolution medium (pH 6.8 phosphate buffer). Samples were analysed using UV spectrophotometer at 276 nm absorbance and % drug release was calculated.
The mechanism of drug release from the tablets was studied by the release data were fitted to zero-order, first-order, Higuchi’s, and Korsmeyer’s Eqns. Zero-order Eqn. is Q=Q0-k0t, where Q is the amount of drug release at time t, and k0 is the release rate; first-order Eqn. is ln Q=ln Q0 -k1t, where k1 is the release rate constant; Higuchi´s Eqn. is Q=k2t1/2, where Q is the amount of drug release at time t, and k2 is the diffusion rate constant [28,29] .
To study any possible interaction between drug and the plant gum, FTIR spectroscopic analysis were carried out. The drug sample, gum and optimized formulation blend i.e. batch F-3 (blend contains gum, diclofenac sodium, prosolv SM, talc and magnesium stearate) compatibility were studied by KBr pellet method using FTIR spectrophotometer (M/s. Perkin– Elmer, 841, Spectrum one). A small amount of diclofenac sodium, gum and formulation blend were respectively placed directly on the germanium piece of the infrared spectrometer with constant pressure applied and infrared absorbance data was collected over the wave number ranged from 4000 cm-1 to 400 cm-1 and was expressed in cm-1  .
Results and Discussion
The current study was undertaken to evaluate the binding potential of L. coromandelica gum using diclofenac sodium was used as model drug in tablets. The granules of different formulation batches prepared (Table 1) were evaluated different parameters like angle of repose (θ), bulk and tapped densities, compressibility index, Hausner’s ratio and the results are shown in Table 2. Angle of repose of all the formulated batches ranges from 29.31 to 34.09, indicating good low properties. Physical characterization of all the batches (Table 3) showing acceptable values of thickness, weight variation (ranged from 199.25±3.76 to 202.50±3.27) was found within limit i.e.±7.5 for the 200 mg tablet as per USP, hardness (ranged from 5-6 kg/cm2), friability (ranged from 0.16 to 0.69 %) within the acceptance range i.e. less than 1%, disintegration time ( <15 min) was found to be acceptable as all the batches and drug content (ranged from 98.05±0.55 to 100.14±1.09%) was that claimed.
In vitro dissolution in phosphate buffer pH 6.8, batch F-3 (containing 10% gum per total weight of tablet) release showed 79.95% of drug releases at the end of 50 min, which is compared with F-7 (containing standard binder povidone at 8% of total weight of tablet) formulation showed 87.23% drug release at the end of 30 min. Batch F-4 (containing 10% of gum) showed 76.53% drug release at the end of 60 min. Batch F-8 released 98.24% drug at the end of 60 min. Hence, it can be concluded that the binding potential of L. coromandelica better as compared to povidone. These results are shown in fig. 1.
All the batches obey zero order kinetics with ‘R2’ values of 0.8910, 0.9407, 0.9954, 0.9969, 0.9386, 0.9818, 0.9502, 0.9869 for F-1, F-2, F-3, F-4, F-5, F-6, F-7 and F-8, respectively. Formulation batch F-5 follow Fickian (Case I) release, as evident from the Table 4. A value of n=0.45 indicates Fickian (case I) release; >0.45 but <0.89 for non-Fickian (anomalous) release; and >0.89 indicates super case II type of release. Case II generally refers to the erosion of the polymeric chain and anomalous transport (non- Fickian) refers to a combination of both diffusion and erosion controlled-drug release.
|Formulation||Zero Order||First Order||Higuchi||Hixson Crowell||Korsmeyer|
Table 4: Release Kinetics Of Various Formulations
Drug-excipient compatibilities studies were carried out by using FTIR spectroscopy. FTIR Spectra of drug (diclofenac sodium) and optimized formulation F-4 (drug, excipients and gum mixture) were analysed. The FTIR spectra of pure diclofenac sodium and its physical mixtures with other excipients are shown in fig. 2 and interpretations were listed in Table 5. The distinct peaks of pure drug were at 770.5, 1045.1, 1284.4, 1574.9, 2968.6, 3424.8 cm-1, while the F4 batch containing L. coromandelica gum as binder showed peaks at 812.4, 996.6, 1280.6,1560.0,2873.6 and 3430.5 cm-1. The distinct peaks are appeared in both pure L. coromandelica gum, indicating no chemical interaction between diclofenac sodium and L. coromandelica gum as confirmed by FTIR. Hence, the gum is safe to use as an excipient in formulation of diclofenac sodium tablets.
|Bonds/Group||Absorption range (cm-1)||Pure Drug (cm-1)||Formulation F-4 (cm-1)||FormulationF-8 (cm-1)|
Table 5: Ftir Data Interpretation
Batch F-3 release showed 77.80% of drug releases at the end of 30 min, which is compared with F-7 formulation, showed 87.23% drug release at the end of 30 min. Hence, it is concluded that L. coromandelica has better binding potential as compared to povidone. Hence, L. coromandelica gum, a natural excipient, can be used as a binder in tablet formulations.
The author expresses his thankfulness to Prof. K. V. Ramana Murthy, A.U. College of Pharmaceutical Sciences, Andhra University, Visakhapatnam (A.P.), India for his valuable advice during the research work. They also thank Crips laboratory Pvt. Ltd. for Diclofenac sodium as gift sample for this work.
Conflict of Interests
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