- *Corresponding Author:
- C. S. Ramaa
Department of Quality Assurance, Bharati Vidyapeeth’s College of Pharmacy, Sector-8, CBD, Navi Mumbai- 400 614, India.
E-mail: [email protected]
|Date of Submission||10 March 2005|
|Date of Revision||27 June 2005|
|Date of Acceptance||31 March 2006|
|Indian J Pharm Sci 2006, 68 (2):265-266|
The objective of this work was to develop a simple, accurate, rapid and sensitive method for the estimation of oxcarbazepine in tablets. The estimation is based on the reduction of ferric ions in its salt form to ferrous ions by the drug, which in presence of potassium ferricyanide produces green colored chromogen measured at 770 nm against reagent blank. The chromogen obeyed linearity over 4 to 28 mg/ml (r=0.9981) with percent relative standard deviation (% RSD) of 0.4267. Recovery studies gave values ranging between 98.26 and 98.76. The proposed method is simple and suitable for routine determination of oxcarbazepine in tablets.
Oxcarbazepine (OXC) is a novel antiepileptic drug, which was developed as a second generation and follow-up compound to carbamazepine (CBZ). OXC has similar therapeutic profile to CBZ but produces much less side effects on patients. It is not official in any pharmacopoeia. Chemically Oxcarbazepine is 10,11-dihydro-10-oxo-5H-dibenz[b,f]azepine-5-carboxamide . Clinically it has been used to treat several types of epilepsy [2-4]. Literature survey reveals determination of oxcarbazepine by chromatographic methods [5-7].
UV/Vis Spectrophotometer 1601 (Shimadzu) with 1 cm matched cuvettes was used for spectrophotometric measurements. Gift sample of oxcarbazepine was received from Glenmark Pharmaceuticals, Mumbai. Tablets of oxcarbazepine were procured from local market. 0.5% ferric chloride and 0.15% potassium ferricyanide in 0.1 N hydrochloric acid solution were freshly prepared. The chemicals were from Loba Chemie.
The standard drug solution (100 μg/ml) was prepared by dissolving 10 mg in acetonitrile making the final volume up to 100 ml with the same solvent. Twenty tablets were powdered. Powder equivalent to 10 mg of oxcarbazepine was extracted with acetonitrile and filtered through Whatman filter paper no. 40. The residue was washed with small quantity of same solvent and the volume was made up to 100 ml.
Aliquots of standard solution, 0.4 to 2.8 ml were transferred to separate series of 10 ml volumetric flasks.
1.4 ml of ferric chloride and 1.2 ml of potassium ferricyanide were added to the respective flasks. The solutions were heated on boiling water bath for 2.0 min and cooled to ambient temperature. After 10 minutes the volume of each flask was adjusted to 10 ml with acetonitrile and the absorbance of solution was measured at 770 nm. Similarly the absorbance of sample solution was measured and the amount of oxcarbazepine was determined by using regression equation referring to the calibration curve.
To test the accuracy and reproducibility of the proposed method, recovery experiment was performed by adding known amount of drug to the reanalyzed formulation and reanalyzing the mixture (Table 1). The recovery was performed at 0%, 100%, 150% and 200% levels.
|Formulation||Label claim (mg/tab)||% Label claim* ± S.D.||% Recovery*1 ± S.D.|
|Tablet 1||150||98.62 ± 0.873||98.97 ± 0.496|
|(Oxecarb, Cipla)||300||99.31 ± 0.996||98.78 ± 0.318|
|Tablet 2||150||99.65 ± 0.483||99.89 ± 0.484|
|(Oxetol, Sun Pharma)||300||99.59 ± 0.571||99.20 ± 1.20|
Table 1: Analysis Data Of Tablet Formulations
The proposed method determines oxcarbazepine in the concentration range of 4–28 μg/ml (r=0.9981) with stability of 1 h. The stability of the colored complex was studied at a concentration of 16 μg/ml of standard solution and adding optimized quantities of ferric chloride (1.4 ml) and potassium ferricyanide (1.2 ml). The time scan analysis was carried out for 2 hrs. The absorbance of this solution was stable at 0.234 for a period of 1 h following which there was gradual decrease in absorbance. The optimum spectrophotometric properties of the colored complex formed with ferric chloride, potassium ferricyanide reagents as well as different parameters affecting color development were extensively studied to determine optimum conditions for assay procedure. The other optical characteristics (Table 2) are as follows: Sandell’s sensitivity is 3.1×10-2 μg/cm2/0.001, the absorptivity of complex is 0.463×104 l/ mole.cm, slope is 0.0182, y-intercept is 0.4297 and relative standard deviation is 0.4567.
|Absorption maxima (nm)||770|
|Beers law limit (µg/ml)||4-28|
|Molar absorptivity (l/mole.cm)||0.463×104|
|Sandell’s sensitivity (µg/cm2/0.001)||3.1×10-2|
|Regression equation (y=mx+c)||0.0182|
|Slope (m) Intercept (c)||0.4297|
|Confidence limit with 95% level||0.0021|
Table 2: Optical Characteristics And Precision
The statistical parameters in method validation studies for precision, accuracy, specificity, stability of analytical solutions and ruggedness have justified the validity of the proposed method. The results of the assay (Table 1) and method validation studies show that the method is simple, accurate and precise with non-interference from tablet excipients.
Thanks are extended to The Principal, Bharati Vidyapeeth’s College of Pharmacy, Sector-8, CBD, Navi Mumbai for his invaluable assistance and encouragement. We are grateful to Glenmark Pharmaceuticals, Mumbai for providing gift sample of oxcarbazepine.
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