RESEARCH PAPER
Year : 2007 | Volume
: 69 | Issue : 2 | Page : 240--243
Development and validation of a HPTLC method for the simultaneous estimation of irbesartan and hydrochlorothiazide in tablet dosage form
NJ Shah1, BN Suhagia2, RR Shah1, NM Patel1
1 Shri B. M. Shah College of Pharmaceutical Education and Research, Modasa - 383 315, India
2 L. M. College of Pharmacy, Navrangpura, Ahmedabad - 380 009, India
Correspondence Address:
N J Shah
Shri B. M. Shah College of Pharmaceutical Education and Research, Modasa - 383 315
India
A simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed and validated for the simultaneous estimation of irbesartan and hydrochlorothiazide in combined dosage forms. The stationary phase used was precoated silica gel 60F[254]. The mobile phase used was a mixture of acetonitrile: chloroform: glacial acetic acid (7:3:0.1 v/v/v). The detection of spots was carried out at 260 nm. The method was validated in terms of linearity, accuracy, precision and specificity. The calibration curve was found to be linear between 100 to 700 ng/spot for irbesartan and 100 to 350 ng/spot for hydrochlorothiazide. The limit of detection and the limit of quantification for the irbesartan were found to be 30 and 100 ng/spot respectively and for hydrochlorothiazide 25 and 100 ng/spot respectively. The proposed method can be successfully used to determine the drug content of marketed formulation.
How to cite this article:
Shah N J, Suhagia B N, Shah R R, Patel N M. Development and validation of a HPTLC method for the simultaneous estimation of irbesartan and hydrochlorothiazide in tablet dosage form.Indian J Pharm Sci 2007;69:240-243
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How to cite this URL:
Shah N J, Suhagia B N, Shah R R, Patel N M. Development and validation of a HPTLC method for the simultaneous estimation of irbesartan and hydrochlorothiazide in tablet dosage form. Indian J Pharm Sci [serial online] 2007 [cited 2013 May 26 ];69:240-243
Available from: http://www.ijpsonline.com/article.asp?issn=0250-474X;year=2007;volume=69;issue=2;spage=240;epage=243;aulast=Shah;type=0 |
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