http://www.ijpsonline.com/currentissue.asp Indian J Pharm Sci 2013 - 75(1) Indian Journal of Pharmaceutical Sciences Medknow Publications0250-474X Rao V. S. V Vadlamudi Editor's note Indian Journal of Pharmaceutical Sciences 2013 75(1):1-2doi:10.4103/0250-474X.113528 Indian Journal of Pharmaceutical Sciences 10.4103/0250-474X.113528 http://www.ijpsonline.com/text.asp?2013/75/1/1/113528 http://www.ijpsonline.com/text.asp?2013/75/1/1/113528751 1 2 http://www.ijpsonline.com/text.asp?2013/75/1/1/113528 Rao V. S. V Vadlamudi

Indian Journal of Pharmaceutical Sciences 2013 75(1):1-2

]]> Mon,17 Jun 2013http://www.ijpsonline.com/text.asp?2013/75/1/1/113528 RR Somani UV Bhanushali Review Article Indian Journal of Pharmaceutical Sciences 2013 75(1):3-10doi:10.4103/0250-474X.113529 Indian Journal of Pharmaceutical Sciences 10.4103/0250-474X.113529 http://www.ijpsonline.com/text.asp?2013/75/1/3/113529 http://www.ijpsonline.com/text.asp?2013/75/1/3/113529751 3 10 http://www.ijpsonline.com/text.asp?2013/75/1/3/113529 RR Somani, UV Bhanushali

Indian Journal of Pharmaceutical Sciences 2013 75(1):3-10

Recent advances in cancer research highlighted the importance of target-specific drug discovery. In view of these advances, the most important mechanism in tumour growth is its ability to stimulate the formation of blood capillaries around itself called tumour-driven angiogenesis. Hence targeting the angiogenesis, inhibits the growth of blood vessels around it and responsible for death of the tumour due to starvation and accumulation of toxic waste. The therapy, thus, indirectly cytotoxic to the tumour cells by targeting newly developing blood vessels. In this review, we summarised the various antiangiogenic agents with their clinical uses and current status.]]> Mon,17 Jun 2013http://www.ijpsonline.com/text.asp?2013/75/1/3/113529 L Bao Y Wang T Shang X Ren R Ma Research Paper Indian Journal of Pharmaceutical Sciences 2013 75(1):11-15doi:10.4103/0250-474X.113531 Indian Journal of Pharmaceutical Sciences 10.4103/0250-474X.113531 http://www.ijpsonline.com/text.asp?2013/75/1/11/113531 http://www.ijpsonline.com/text.asp?2013/75/1/11/113531751 11 15 http://www.ijpsonline.com/text.asp?2013/75/1/11/113531 L Bao, Y Wang, T Shang, X Ren, R Ma

Indian Journal of Pharmaceutical Sciences 2013 75(1):11-15

Hospital information system is widely used to improve work efficiency of hospitals in China. However, it is lack of the function providing pharmaceutical information service for clinical pharmacists. A novel clinical pharmacy management system developed by our hospital was introduced to improve the work efficiency of clinical pharmacists in our hospital and to carry out large sample statistical analyzes by providing pharmacy information services and promoting rational drug use. Clinical pharmacy management system was developed according to the actual situation. Taking prescription review in the department of general surgery as the example, work efficiency of clinical pharmacists, quality and qualified rates of prescriptions before and after utilizing clinical pharmacy management system were compared. Statistics of 48,562 outpatient and 5776 inpatient prescriptions of the general surgical department were analyzed. Qualified rates of both the inpatient and outpatient prescriptions of the general surgery department increased, and the use of antibiotics decreased. This system apparently improved work efficiency, standardized the level and accuracy of drug use, which will improve the rational drug use and pharmacy information service in our hospital. Meanwhile, utilization of prophylactic antibiotics for the aseptic operations also reduced.]]> Mon,17 Jun 2013http://www.ijpsonline.com/text.asp?2013/75/1/11/113531 N Tyagi SS Rathore PC Ghosh Research Paper Indian Journal of Pharmaceutical Sciences 2013 75(1):16-22doi:10.4103/0250-474X.113533 Indian Journal of Pharmaceutical Sciences 10.4103/0250-474X.113533 http://www.ijpsonline.com/text.asp?2013/75/1/16/113533 http://www.ijpsonline.com/text.asp?2013/75/1/16/113533751 16 22 http://www.ijpsonline.com/text.asp?2013/75/1/16/113533 N Tyagi, SS Rathore, PC Ghosh

Indian Journal of Pharmaceutical Sciences 2013 75(1):16-22

The monensin, known to enhance the cytotoxicity of ricin and ricin-based immunotoxins is a very hydrophobic molecule and this limits its administration in optimum doses under in vivo conditions. In order to realise its full potential, monensin was intercalated into various liposomal formulations and its ability to potentiate the cytotoxicity of ricin liposomes in human epidermoid carcinoma (KB) cells was studied. It was observed that ricin cytotoxicity enhancing ability of monensin liposome depends on the surface charge as well as density and chain length of distearoyl phosphatidylethanolamine-methoxy polyethylene glycol present on the surface of liposomal monensin. Maximum potentiation on the cytotoxicity of liposomal ricin was observed by monensin entrapped in neutral liposome (106.5 fold) followed by negatively charged (94.2 fold) and positively charged liposome (90 fold). Studies on the effect of variation of density and chain length of distearoyl phosphatidylethanolamine-methoxy polyethylene glycol showed that neutral monensin liposomes having 2.5 mol% distearoyl phosphatidylethanolamine-methoxy polyethylene glycol with chain length of 2000 exhibits maximum potentiation (117.6 fold) on the cytotoxicity of ricin liposomes when the cellular uptake of monensin liposome was maximum (42.0%) and the zeta potential value on the surface of liposomes was −0.645. The present study has clearly shown that liposomal monensin is very effective in enhancing the cytotoxicity of liposomal ricin in human cancer cells and liposome can be used as in vivo deliver vehicle for monensin to potentiate the cytotoxicity of liposomal ricin to eliminate cancer cells.]]> Mon,17 Jun 2013http://www.ijpsonline.com/text.asp?2013/75/1/16/113533 Meenakshi Sindhu Vandana Saini Sakshi Piplani A Kumar Research Paper Indian Journal of Pharmaceutical Sciences 2013 75(1):23-30doi:10.4103/0250-474X.113534 Indian Journal of Pharmaceutical Sciences 10.4103/0250-474X.113534 http://www.ijpsonline.com/text.asp?2013/75/1/23/113534 http://www.ijpsonline.com/text.asp?2013/75/1/23/113534751 23 30 http://www.ijpsonline.com/text.asp?2013/75/1/23/113534 Meenakshi Sindhu, Vandana Saini, Sakshi Piplani, A Kumar

Indian Journal of Pharmaceutical Sciences 2013 75(1):23-30

The structure-function correlation of membrane proteins have been a difficult task, particularly in context to transient protein complexes. The molecular simulation of ternary complex of Rab7::REP1::GGTase-II was carried out to understand the basic structural events occurring during the prenylation event of Rab proteins, using the software YASARA. The study suggested that the C-terminus of Rab7 has to be in completely extended conformation during prenylation to reach the active site of RabGGTase-II. Also, attempt was made to find putative drug binding sites on the ternary complex of Rab7::REP1::GGTase-II using Q-SiteFinder programme. The comprehensive consensus probe generated by the program revealed a total of 10 major pockets as putative drug binding sites on Rab7::REP:: GGTase-II ternary complex. These pockets were found on REP protein and GGTase protein subunits. The Rab7 was found to be devoid of any putative drug binding sites in the ternary complex. The phylogenetic analysis of 60 Rab proteins of human was carried out using PHYLIP and study indicated the close phylogenetic relationship between Rab7 and Rab9 proteins of human and hence with further in silico study, the present observations can be extrapolated to Rab9 proteins. The study paves a good platform for further experimental verifications of the findings and other in silico studies like identifying the potential drug targets by searching the putative drug binding sites, generating pharmacophoric pattern, searching or constructing suitable ligand and docking studies.]]> Mon,17 Jun 2013http://www.ijpsonline.com/text.asp?2013/75/1/23/113534 DR Acharya Dipti B Patel Research Paper Indian Journal of Pharmaceutical Sciences 2013 75(1):31-35doi:10.4103/0250-474X.113538 Indian Journal of Pharmaceutical Sciences 10.4103/0250-474X.113538 http://www.ijpsonline.com/text.asp?2013/75/1/31/113538 http://www.ijpsonline.com/text.asp?2013/75/1/31/113538751 31 35 http://www.ijpsonline.com/text.asp?2013/75/1/31/113538 DR Acharya, Dipti B Patel

Indian Journal of Pharmaceutical Sciences 2013 75(1):31-35

A simple, accurate, rapid and precise reversed-phase high-performance liquid chromatographic method has been developed and validated for simultaneous determination of cefpodoxime proxetil and dicloxacillin sodium in tablet. The chromatographic separation was carried out on kromasil C 18 analytical column (250×4.6 mm; 5 μm) with a mixture of acetonitrile:methanol:trifloroacetic acid (0.001%) with pH 6.5 (30:50:20, v/v/v) as mobile phase; at a flow rate of 1.0 ml/min. UV detection was performed at 235 nm. The dicloxacillin sodium and cefpodoxime proxetil were eluted at 1.92 and 3.35 min, respectively. The peaks were eluted with better resolution. Calibration plots were linear over the concentration range 0.5-20 μg/ml for cefpodoxime proxetil (r 2=0.9996) and 5-50 μg/ml for dicloxacillin sodium (r 2=0.9987). The method was validated for accuracy, precision, linearity and specificity. The method was very sensitive with limit of detection 0.0726, 0.3685 μg/ml and limit of quantification 0.220, 1.116 μg/ml for cefpodoxime proxetil and dicloxacillin sodium, respectively. The high recovery and low relative standard deviation confirm the suitability of the method for routine determination of cefpodoxime proxetil and dicloxacillin sodium in bulk drug and tablet dosage form.]]> Mon,17 Jun 2013http://www.ijpsonline.com/text.asp?2013/75/1/31/113538 P Singh Research Paper Indian Journal of Pharmaceutical Sciences 2013 75(1):36-44doi:10.4103/0250-474X.113539 Indian Journal of Pharmaceutical Sciences 10.4103/0250-474X.113539 http://www.ijpsonline.com/text.asp?2013/75/1/36/113539 http://www.ijpsonline.com/text.asp?2013/75/1/36/113539751 36 44 http://www.ijpsonline.com/text.asp?2013/75/1/36/113539 P Singh

Indian Journal of Pharmaceutical Sciences 2013 75(1):36-44

The tumour necrosis factor-α converting enzyme inhibition activity of a series comprising of novel tartrate-based analogues has been quantitatively analysed in terms of molecular descriptors. The statistically validated quantitative structure-activity relationship models provided rationales to explain the inhibition activity of these congeners. The descriptors identified through combinatorial protocol in multiple linear regression analysis have highlighted the role of Moran autocorrelation of lag 7, weighted by atomic van der Waals volume, presence of both prime and nonprime amide carbonyl oxygen in the tartrate moiety and occurrence of five membered ring bearing substituents at varying sites. A few potential novel tartrate-based analogues have been suggested for further investigation.]]> Mon,17 Jun 2013http://www.ijpsonline.com/text.asp?2013/75/1/36/113539 PF Builders B Kabele-Toge M Builders BA Chindo Patricia A Anwunobi Yetunde C Isimi Research Paper Indian Journal of Pharmaceutical Sciences 2013 75(1):45-52doi:10.4103/0250-474X.113549 Indian Journal of Pharmaceutical Sciences 10.4103/0250-474X.113549 http://www.ijpsonline.com/text.asp?2013/75/1/45/113549 http://www.ijpsonline.com/text.asp?2013/75/1/45/113549751 45 52 http://www.ijpsonline.com/text.asp?2013/75/1/45/113549 PF Builders, B Kabele-Toge, M Builders, BA Chindo, Patricia A Anwunobi, Yetunde C Isimi

Indian Journal of Pharmaceutical Sciences 2013 75(1):45-52

Wound healing agents support the natural healing process, reduce trauma and likelihood of secondary infections and hasten wound closure. The wound healing activities of water in oil cream of the methanol extract of Hibiscus sabdariffa L. (Malvaceae) was evaluated in rats with superficial skin excision wounds. Antibacterial activities against Pseudomonas aeroginosa, Staphylococcus aureus and Echerichia coli were determined. The total flavonoid content, antioxidant properties and thin layer chromatographic fingerprints of the extract were also evaluated. The extract demonstrated antioxidant properties with a total flavonoid content of 12.30±0.09 mg/g. Six reproducible spots were obtained using methanol:water (95:5) as the mobile phase. The extract showed no antimicrobial activity on the selected microorganisms, which are known to infect and retard wound healing. Creams containing H. sabdariffa extract showed significant (P<0.05) and concentration dependent wound healing activities. There was also evidence of synergism with creams containing a combination of gentamicin and H. sabdariffa extract. This study, thus, provides evidence of the wound healing potentials of the formulated extract of the calyces of H. sabdariffa and synergism when co-formulated with gentamicin.]]> Mon,17 Jun 2013http://www.ijpsonline.com/text.asp?2013/75/1/45/113549 R Lakshmi E James R Kirthivasan Research Paper Indian Journal of Pharmaceutical Sciences 2013 75(1):53-59doi:10.4103/0250-474X.113550 Indian Journal of Pharmaceutical Sciences 10.4103/0250-474X.113550 http://www.ijpsonline.com/text.asp?2013/75/1/53/113550 http://www.ijpsonline.com/text.asp?2013/75/1/53/113550751 53 59 http://www.ijpsonline.com/text.asp?2013/75/1/53/113550 R Lakshmi, E James, R Kirthivasan

Indian Journal of Pharmaceutical Sciences 2013 75(1):53-59

Anticoagulants are very useful medications but can also lead to haemorrhagic as well as thromboembolic complications when not used correctly or without proper medical attention. Anticoagulant's complex pharmacology and pharmacokinetics contribute to its narrow margin of safety. Pharmacist's unique knowledge of pharmacology, pharmacokinetics and interactions makes them well-suited to assist patients in maintaining safe and effective anticoagulation. Successful anticoagulation therapy implies fewer incidences of therapeutic failures and bleeding complications. The anticoagulation management service staffed by clinical pharmacists is a service established to monitor and manage oral and parenteral anticoagulants. In this research work, 40 patients each were included in the intervention and the control groups. In the intervention group, patient's knowledge score on anticoagulation increased from an average of 5.6±3.2 to 13.8±0.94 (P=0.000) after clinical pharmacist's counselling, whereas in the control group there was no significant improvement in patient's baseline knowledge over the knowledge score at the end of the study (8.0±1.59 vs. 8.3±2.6) (P=0.218). In the intervention group, 73.45% of the international normalised ratio test results were within the therapeutic range, 8.45% supratherapeutic and 18.5% subtherapeutic during the 6 months data collection period. The corresponding data for the control group were 53.2 (P=0.000), 18.4 (P=0.000) and 28.4% (P=0.002), respectively. Forty four adverse drug reactions (ADRs) related to anticoagulants were identified in the intervention group as compared to 56 in the control group. These results revealed that the clinical pharmacist's involvement in the anticoagulation management improved the therapeutic outcome of patients and demonstrate the benefits of clinical pharmacist guided anticoagulation clinics in India.]]> Mon,17 Jun 2013http://www.ijpsonline.com/text.asp?2013/75/1/53/113550 RD Jangle BN Thorat Research Paper Indian Journal of Pharmaceutical Sciences 2013 75(1):60-66doi:10.4103/0250-474X.113555 Indian Journal of Pharmaceutical Sciences 10.4103/0250-474X.113555 http://www.ijpsonline.com/text.asp?2013/75/1/60/113555 http://www.ijpsonline.com/text.asp?2013/75/1/60/113555751 60 66 http://www.ijpsonline.com/text.asp?2013/75/1/60/113555 RD Jangle, BN Thorat

Indian Journal of Pharmaceutical Sciences 2013 75(1):60-66

A simple, sensitive, precise and specific method for the determination of curcuminoids and curcuminoid-loaded liposome formulation was developed using reverse-phase high-performance liquid chromatography method. The analysis was performed isocratically on Zorbax Eclipse XDB-C18 column (150×4 mm, 5 μm), analytical column using UV detector and mobile phase consisting of 0.1% orthophosphoric acid and acetonitrile. The proposed method for curcuminoids was validated for linearity in the range from 50 to 300 ΅g/ml with correlation coefficient above 0.997. Intraday and interday precision studies showed the relative standard deviation less than 2%. The limit of detection and limit of quantitation values were 2.5 and 8.25 ΅g/ml, respectively. Forced degradation study for curcuminoids and liposomal curcuminoids sample was carried out and observed that proposed method was also suitable for finding degradation products in the sample. Proposed method was successfully applied to estimate curcuminoids content without any interference of other excipients from liposomal formulation. Therefore, the method developed is well suited for curcuminoids and its liposome estimation.]]> Mon,17 Jun 2013http://www.ijpsonline.com/text.asp?2013/75/1/60/113555 J Sruti Ch N Patra SK Swain S Beg HR Palatasingh SC Dinda M.E Bhanoji Rao Research Paper Indian Journal of Pharmaceutical Sciences 2013 75(1):67-75doi:10.4103/0250-474X.113551 Indian Journal of Pharmaceutical Sciences 10.4103/0250-474X.113551 http://www.ijpsonline.com/text.asp?2013/75/1/67/113551 http://www.ijpsonline.com/text.asp?2013/75/1/67/113551751 67 75 http://www.ijpsonline.com/text.asp?2013/75/1/67/113551 J Sruti, Ch N Patra, SK Swain, S Beg, HR Palatasingh, SC Dinda, M.E Bhanoji Rao

Indian Journal of Pharmaceutical Sciences 2013 75(1):67-75

A combination of fusion and surface adsorption techniques was used to enhance the dissolution rate of cefuroxime axetil. Solid dispersions of cefuroxime axetil were prepared by two methods, namely fusion method using poloxamer 188 alone and combination of poloxamer 188 and Neusilin US2 by fusion and surface adsorption method. Solid dispersions were evaluated for solubility, phase solubility, flowability, compressibility, Kawakita analysis, Fourier transform-infrared spectra, differential scanning calorimetry, powder X-ray diffraction study, in vitro drug release, and stability study. Solubility studies showed 12- and 14-fold increase in solubility for solid dispersions by fusion method, and fusion and surface adsorption method, respectively. Phase solubility studies showed negative values for poloxamer 188 at various concentrations (0, 0.25, 0.5, 0.75 and 1%) indicating spontaneous nature of solubilisation. Fourier transform-infrared spectra and differential scanning calorimetry spectra showed that drug and excipients are compatible with each other. Powder X-ray diffraction study studies indicated that presence of Neusilin US2 is less likely to promote the reversion of the amorphous cefuroxime axetil to crystalline state. In vitro dissolution studies, T50% and mean dissolution time have shown better dissolution rate for solid dispersions by fusion and surface adsorption method. Cefuroxime axetil release at 15 min (Q15) and DE15 exhibited 23- and 20-fold improvement in dissolution rate. The optimized solid dispersion formulation was stable for 6 months of stability study as per ICH guidelines. The stability was ascertained from drug content, in vitro dissolution, Fourier transform-infrared spectra and differential scanning calorimetry study. Hence, this combined approach of fusion and surface adsorption can be used successfully to improve the dissolution rate of poorly soluble biopharmaceutical classification system class II drug cefuroxime axetil.]]> Mon,17 Jun 2013http://www.ijpsonline.com/text.asp?2013/75/1/67/113551 N Sharma SS Rao B Vaghela Research Paper Indian Journal of Pharmaceutical Sciences 2013 75(1):76-82doi:10.4103/0250-474X.113530 Indian Journal of Pharmaceutical Sciences 10.4103/0250-474X.113530 http://www.ijpsonline.com/text.asp?2013/75/1/76/113530 http://www.ijpsonline.com/text.asp?2013/75/1/76/113530751 76 82 http://www.ijpsonline.com/text.asp?2013/75/1/76/113530 N Sharma, SS Rao, B Vaghela

Indian Journal of Pharmaceutical Sciences 2013 75(1):76-82

The objective of current investigation was to study the degradation behaviour of eberconazole nitrate and mometasone furoate under different International Conference on harmonisation recommended stress condition using reverse phase high performance liquid chromatographic method and to establish validated stability-indicating high performance liquid chromatographic method to determine purity of eberconazole nitrate and mometasone furoate in presence of its impurities, forced degradation products and placebo in pharmaceutical dosage forms. The method was developed using Hypersil BDS, C18, 150Χ4.6 mm, 5 μ as stationary phase with mobile phase containing a gradient mixture of solvent A and B. 0.01 M phosphate buffer with 0.1% triethyl amine, adjusted pH 7.0 with phosphoric acid was used as buffer. Buffer pH 7.0 was used as solvent A and methanol:acetonitrile in 150:850 v/v ratios were used as solvent B. The eluted compounds were monitored at 240 nm. The run time was 50 min. The developed method was validated as per international conference on harmonization guidelines with respect to specificity, linearity, limit of detection, limit of quantification, accuracy, precision and robustness.]]> Mon,17 Jun 2013http://www.ijpsonline.com/text.asp?2013/75/1/76/113530 Shruti Srivastava P Singh KK Jha Garima Mishra S Srivastava RL Khosa Research Paper Indian Journal of Pharmaceutical Sciences 2013 75(1):83-88doi:10.4103/0250-474X.113532 Indian Journal of Pharmaceutical Sciences 10.4103/0250-474X.113532 http://www.ijpsonline.com/text.asp?2013/75/1/83/113532 http://www.ijpsonline.com/text.asp?2013/75/1/83/113532751 83 88 http://www.ijpsonline.com/text.asp?2013/75/1/83/113532 Shruti Srivastava, P Singh, KK Jha, Garima Mishra, S Srivastava, RL Khosa

Indian Journal of Pharmaceutical Sciences 2013 75(1):83-88

In the present study, methanol extracts of Costus speciosus Koen. aerial parts were assessed for antiinflammatory, analgesic and antipyretic activities in experimental animals. The antiinflammatory activity of methanol extract of Costus speciosus (400 and 800 mg/kg, p.o.) was evaluated using carrageenan-induced paw oedema test. Analgesic effect was evaluated using acetic acid-induced writhing and Eddy's hot-plate models and antipyretic activity was assessed by Brewer's yeast-induced pyrexia in rats. The methanol extract of aerial parts of Costus speciosus in a dose of 400 and 800 mg/kg showed significant antiinflammatory activity (19.36 and 40.05% reduction) at 5 h postmedication. In analgesic models extract treated animals at (400 and 800 mg/kg) inhibited writhing's caused by acetic acid by 14.24 and 31.90%, respectively, and it also increased the latency period at both high and low doses which showed the mean reaction time at 16.60±0.355 s and 14.12±0.355 s, respectively, when compared to control in hot-plate test. It also reduces the rectal temperature of the animals at low and high doses significantly 37.03±0.108° and 36.63±0.098°, respectively, in Brewer's yeast induced pyrexia. The obtained results of the present investigation revealed that methanol extract of Costus speciosus has significant antiinflammatory, analgesic and antipyretic activities.]]> Mon,17 Jun 2013http://www.ijpsonline.com/text.asp?2013/75/1/83/113532 SK Dubey S Duddelly H Jangala RN Saha Research Paper Indian Journal of Pharmaceutical Sciences 2013 75(1):89-93doi:10.4103/0250-474X.113535 Indian Journal of Pharmaceutical Sciences 10.4103/0250-474X.113535 http://www.ijpsonline.com/text.asp?2013/75/1/89/113535 http://www.ijpsonline.com/text.asp?2013/75/1/89/113535751 89 93 http://www.ijpsonline.com/text.asp?2013/75/1/89/113535 SK Dubey, S Duddelly, H Jangala, RN Saha

Indian Journal of Pharmaceutical Sciences 2013 75(1):89-93

A reliable, rapid and sensitive isocratic reverse phase high-performance liquid chromatography method has been developed and validated for assay of ketorolac tromethamine in tablets and ophthalmic dosage forms using diclofenac sodium as an internal standard. An isocratic separation of ketorolac tromethamine was achieved on Oyster BDS (150×4.6 mm i.d., 5 μm particle size) column using mobile phase of methanol:acetonitrile:sodium dihydrogen phosphate (20 mM; pH 5.5) (50:10:40, %v/v) at a flow rate of 1.0 ml/min. The eluents were monitored at 322 nm for ketorolac and at 282 nm for diclofenac sodium with a photodiode array detector. The retention times of ketorolac and diclofenac sodium were found to be 1.9 min and 4.6 min, respectively. Response was a linear function of drug concentration in the range of 0.01-15 μg/ml (R2 =0.994; linear regression model using weighing factor 1/x2 ) with a limit of detection and quantification of 0.002 μg/ml and 0.007 μg/ml, respectively. The % recovery and % relative standard deviation values indicated the method was accurate and precise.]]> Mon,17 Jun 2013http://www.ijpsonline.com/text.asp?2013/75/1/89/113535 RA Xu ZS Xu GY Lin LF Hu XQ Wang JS Ma SHORT COMMUNICATION Indian Journal of Pharmaceutical Sciences 2013 75(1):94-98doi:10.4103/0250-474X.113536 Indian Journal of Pharmaceutical Sciences 10.4103/0250-474X.113536 http://www.ijpsonline.com/text.asp?2013/75/1/94/113536 http://www.ijpsonline.com/text.asp?2013/75/1/94/113536751 94 98 http://www.ijpsonline.com/text.asp?2013/75/1/94/113536 RA Xu, ZS Xu, GY Lin, LF Hu, XQ Wang, JS Ma

Indian Journal of Pharmaceutical Sciences 2013 75(1):94-98

Wuniu early tea (Camellia sinensis) is an important beverage consumed in China. Up to date, a lot of methods for identifying and chemical analysing have been done. However, there is no report on the effects of Wuniu early tea on cytochrome P450 isozymes. Therefore, the present objective of our study was to evaluate the potential effects of Wuniu early tea on cytochrome P450 isozymes P2C9, P1A2, P2C19 and P2B6 in rats with a cocktail approach including, matching probe drugs of tolbutamide, phenacetin, omeprazole and bupropion. These four probe drugs were simultaneously administered to rats after repeated Wuniu early tea administration. The pharmacokinetics of the probes in the plasma was simultaneous determined by high-performance liquid chromatography-mass spectrometry. The t 1/2 and AUC (0-∞) of tolbutamide increased significantly and CL z decreased remarkably in test rats after repeated Wuniu early tea administration. However, the main pharmacokinetic parameters of the other three probe drugs were not significantly different between control and test rats. The findings in this study suggested that Wuniu early tea could inhibit cytochrome P2C9 while did not influence on cytochrome P1A2, cytochrome P2C19 and cytochrome P2B6.]]> Mon,17 Jun 2013http://www.ijpsonline.com/text.asp?2013/75/1/94/113536 Anuradha Rai SHORT COMMUNICATION Indian Journal of Pharmaceutical Sciences 2013 75(1):99-102doi:10.4103/0250-474X.113537 Indian Journal of Pharmaceutical Sciences 10.4103/0250-474X.113537 http://www.ijpsonline.com/text.asp?2013/75/1/99/113537 http://www.ijpsonline.com/text.asp?2013/75/1/99/113537751 99 102 http://www.ijpsonline.com/text.asp?2013/75/1/99/113537 Anuradha Rai

Indian Journal of Pharmaceutical Sciences 2013 75(1):99-102

The ethanol Hedera helix plant extract was tested for its antiinflammatory properties. Intraperitoneal injections of 7.5 ml/kg wt ethanol extract showed antiinflammatory activity with 88.89% inhibition as compared to reference drug diclofenac, which showed 94.44% inhibition in formalin-induced paw oedema. As formalin-induced paw oedema closely resembles human arthritis, the antiarthritic property of ethanol extract of Hedera helix was also investigated. The visible reduction in arthritic symptoms by extract of Hedera helix suggests the potential of the plant extract against inflammation and arthritis.]]> Mon,17 Jun 2013http://www.ijpsonline.com/text.asp?2013/75/1/99/113537 D Wrzesniok M Otreba A Beberok E Buszman SHORT COMMUNICATION Indian Journal of Pharmaceutical Sciences 2013 75(1):102-106doi:10.4103/0250-474X.113540 Indian Journal of Pharmaceutical Sciences 10.4103/0250-474X.113540 http://www.ijpsonline.com/text.asp?2013/75/1/102/113540 http://www.ijpsonline.com/text.asp?2013/75/1/102/113540751 102 106 http://www.ijpsonline.com/text.asp?2013/75/1/102/113540 D Wrzesniok, M Otreba, A Beberok, E Buszman

Indian Journal of Pharmaceutical Sciences 2013 75(1):102-106

Aminoglycosides, such as amikacin and kanamycin, are powerful broad-spectrum antibiotics used for the treatment of many bacterial infections. The widely used aminoglycosides have the unfortunate side effects of targeting sensory hair cells of the inner ear, so that treatment often results in permanent hair cell loss. The aim of the study was to evaluate the influence of incubation time and drug concentration on viability of melanocytes cultured in the presence of amikacin or kanamycin. The normal human melanocytes HEMa-LP and the different concentrations of amikacin (0.075, 0.75 and 7.5 mmol/l) and kanamycin (0.06, 0.6 and 6.0 mmol/l), were used. The estimations were performed after 24, 48 and 72 h. The observed decrease in melanocytes viability may be an explanation for the mechanisms involved in aminoglycosides toxicity on pigmented tissues during high-dose and/or long-term therapy.]]> Mon,17 Jun 2013http://www.ijpsonline.com/text.asp?2013/75/1/102/113540 VR Patel RK Patel SHORT COMMUNICATION Indian Journal of Pharmaceutical Sciences 2013 75(1):106-109doi:10.4103/0250-474X.113541 Indian Journal of Pharmaceutical Sciences 10.4103/0250-474X.113541 http://www.ijpsonline.com/text.asp?2013/75/1/106/113541 http://www.ijpsonline.com/text.asp?2013/75/1/106/113541751 106 109 http://www.ijpsonline.com/text.asp?2013/75/1/106/113541 VR Patel, RK Patel

Indian Journal of Pharmaceutical Sciences 2013 75(1):106-109

Manjisthadi churna has been traditionally used in the Ayurvedic system of medicine and by traditional medical practices of India to treat hyperlipidemia. A rapid, simple and accurate method with high performance thin layer chromatography has been developed to standardised Manjisthadi churna using rubiadin, sennoside and ellagic acid as markers. Methanol extract of Manjisthadi churna were used for high performance thin layer chromatography on silica gel plates. The Rf of rubiadin, sennoside-A and ellagic acid were found to 0.48, 0.23 and 0.72, respectively with densitometric scanning at 280 nm and the calibration plot were linear in the range of 100-600 ng of markers. The correlation coefficients were higher than 0.99 were indicative of good linear dependence of peaks area on concentration. The rubiadin, sennoside-A and ellagic acid contents in Manjisthadi churna were found to be 0.014, 0.038 and 0.534% w/w, respectively. This method permits reliable quantification of rubiadin, sennoside-A and ellagic acid with good resolution and separation of the same from other constitutes of the extract of Manjisthadi churna. Recovery value from 95.66-102.33% showed the reliability and reproducibility of the method. The proposed high performance thin layer chromatography method for simultaneous quantification of markers in Manjisthadi churna can be used for routine quality testing.]]> Mon,17 Jun 2013http://www.ijpsonline.com/text.asp?2013/75/1/106/113541 M Arivalagan KK Gangopadhyay G Kumar SHORT COMMUNICATION Indian Journal of Pharmaceutical Sciences 2013 75(1):110-113doi:10.4103/0250-474X.113542 Indian Journal of Pharmaceutical Sciences 10.4103/0250-474X.113542 http://www.ijpsonline.com/text.asp?2013/75/1/110/113542 http://www.ijpsonline.com/text.asp?2013/75/1/110/113542751 110 113 http://www.ijpsonline.com/text.asp?2013/75/1/110/113542 M Arivalagan, KK Gangopadhyay, G Kumar

Indian Journal of Pharmaceutical Sciences 2013 75(1):110-113

Trigonella foenum-graecum, commonly called fenugreek, is a leguminous plant native to many Asian, Middle Eastern and European countries. Fenugreek oil is very effective in digestion. Identification of fenugreek genotype rich in saponins and fixed oil will be useful for pharmaceutical industries. In the present study, steroidal saponin and fixed oil content was analysed in 46 diverse fenugreek genotypes on dry weight basis. Significant differences were observed in the total saponin and fixed oil content among the genotypes. Saponin and fixed oil content ranged from 0.92 g to 1.68 g and 3.25 to 6.88 g with corresponding mean value of 1.34 g and 5.19 g/100 g dw, respectively.]]> Mon,17 Jun 2013http://www.ijpsonline.com/text.asp?2013/75/1/110/113542 T Anand GK Ingle J Kishore R Kumar SHORT COMMUNICATION Indian Journal of Pharmaceutical Sciences 2013 75(1):113-117doi:10.4103/0250-474X.113543 Indian Journal of Pharmaceutical Sciences 10.4103/0250-474X.113543 http://www.ijpsonline.com/text.asp?2013/75/1/113/113543 http://www.ijpsonline.com/text.asp?2013/75/1/113/113543751 113 117 http://www.ijpsonline.com/text.asp?2013/75/1/113/113543 T Anand, GK Ingle, J Kishore, R Kumar

Indian Journal of Pharmaceutical Sciences 2013 75(1):113-117

A matrix based on coupling of cost (always, better and control) analysis and criticality (vital, essential and desirable) analysis was employed for drug inventory containing 129 items of drug store in the Department of Community Medicine of a Medical College in Delhi. The annual drug expenditure incurred on 129 drug items for the year 2010-2011 was found to be Rs. 4,35,847.85. On always, better and control analysis, 18.6, 24.0 and 57.4% drugs were found to be always, better and control category items, respectively, amounting for 69.1, 20.8 and 10.1% of annual drug expenditure. About 13.2 (17), 38.8 (50) and 48.0% (62) items were found to be vital, essential and desirable category items, respectively, amounting for 18.7, 49.5 and 31.8% of annual drug expenditure. Based on always, better and control-vital, essential and desirable matrix analysis there were 37 (28.68%) items in category I, 53 (41.09%) items in category II and 39 (30.23%) items in category III, amounting for 73.0, 22.2 and 4.8% of annual drug expenditure, respectively. To conclude, scientific inventory management tools are needed to be applied in routine for efficient management of the pharmacy stores as it contributes to not only in improvement in patient care but also judicious use of resources as well.]]> Mon,17 Jun 2013http://www.ijpsonline.com/text.asp?2013/75/1/113/113543 Z Parveen S Nawaz S Siddique K Shahzad SHORT COMMUNICATION Indian Journal of Pharmaceutical Sciences 2013 75(1):117-122doi:10.4103/0250-474X.113544 Indian Journal of Pharmaceutical Sciences 10.4103/0250-474X.113544 http://www.ijpsonline.com/text.asp?2013/75/1/117/113544 http://www.ijpsonline.com/text.asp?2013/75/1/117/113544751 117 122 http://www.ijpsonline.com/text.asp?2013/75/1/117/113544 Z Parveen, S Nawaz, S Siddique, K Shahzad

Indian Journal of Pharmaceutical Sciences 2013 75(1):117-122

The essential oil from the leaves of Curcuma longa L. Kasur variety grown in Pakistan was extracted by hydro-distillation. Chemical constituents of the essential oil were identified by gas chromatography/mass spectrometry. The chromatographic analysis of oil showed 25 constituents, out of which nine chemical constituents were identified. The eucalyptol (10.27%) was the major component of the essential oil. α-pinene (1.50%), β-phellandrene (2.49%), β-pinene (3.57%), limonene (2.73%), 1,3,8-p-menthatriene (1.76%), ascaridole epoxide (1.452%), 2-methylisoborneol (2.92%), 5-isopropyl-6-methyl-hepta-3, dien-2-ol (2.07%) were also present in considerable quantity. The antimicrobial properties of leaves of Curcuma longa were tested by disc diffusion method against various human pathogens, including eight fungal and five bacterial strains. Essential oil showed maximum resistance against Fusarium miniformes MAY 3629 followed by Bacillus subtilis ATCC 6633 whereas; it exhibited least resistance against Fusarium oxysporium ATCC 48122. The results of the antimicrobial assay revealed that essential oil showed significant inhibitory activity against the tested organisms.]]> Mon,17 Jun 2013http://www.ijpsonline.com/text.asp?2013/75/1/117/113544 T Murugan J Albino Wins M Murugan SHORT COMMUNICATION Indian Journal of Pharmaceutical Sciences 2013 75(1):122-125doi:10.4103/0250-474X.113546 Indian Journal of Pharmaceutical Sciences 10.4103/0250-474X.113546 http://www.ijpsonline.com/text.asp?2013/75/1/122/113546 http://www.ijpsonline.com/text.asp?2013/75/1/122/113546751 122 125 http://www.ijpsonline.com/text.asp?2013/75/1/122/113546 T Murugan, J Albino Wins, M Murugan

Indian Journal of Pharmaceutical Sciences 2013 75(1):122-125

Plants produce a wide variety of phytochemical constituents, which are secondary metabolites and are used either directly or indirectly in the pharmaceutical industry. 'For centuries, man has effectively used various components of plants or their extracts for the treatment of many diseases, including bacterial infections. In the present study methanol, chloroform and aqueous extracts of Cassia auriculata leaf were subjected for antimicrobial activity by well-diffusion method against six bacterial strains namely Bacillus cereus, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Proteus mirabilis. The results revealed that the methanol and chloroform extracts exhibited strong inhibitory activity against all the tested organisms (zone of inhibition of 12-20 mm), except Pseudomonas aeruginosa (zone of inhibition 10 mm or nil). The aqueous extracts showed moderate activity by 'Zone of inhibition ≤12 or nil). The extracts were screened for their phytochemical constituents by standard protocols' and were shown to contain carbohydrates, proteins, alkaloids, flavonoids, steroids, saponins and tannins. The antibacterial activity of these extracts is possibly linked to the presence of flavonoids, steroid, saponins and/or tannins. Further studies are needed to determine the precise active principles from Cassia auriculata.]]> Mon,17 Jun 2013http://www.ijpsonline.com/text.asp?2013/75/1/122/113546