*Corresponding Author:
Shajidul Karim
Department of Pharmaceutical Analysis and Quality Assurance, Himalayan Pharmacy Institute, Majitar, Sikkim 737136, India
E-mail: shajidulkarim451@gmail.com
Date of Received 10 March 2020
Date of Revision 6 March 2023
Date of Acceptance 8 August 2024
Indian J Pharm Sci 2024;86(4):1296-1302  

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Abstract

Evogliptin is an anti-diabetic drug, which comes under the class of gliptin derivatives for the inhibition of selective dipeptidyl peptidase-4 inhibitor. The developed ultraviolet spectrophotometric method was simple, sensitive, accurate, precise and economic for the development and validation of evogliptin in bulk and tablet dosage form. In this present study, the analytical method validation and development of evogliptin was done using the different parameters of method validation as per International Council for Harmonisation Q2(R1) guidelines. Water using as a solvent and it shows the maximum wavelength at 266 nm and then performed all the parameters of analytical method validation like accuracy, precision, linearity, range, robustness, ruggedness, limit of detection and limit of quantitation. Evogliptin showed linearity over the range of 2-48 µg/ml. The correlation coefficient value obtained was 0.996 with the regression equation y=0.0032x+0.0005. The accuracy studies was done in spiking method and the recoveries ranging from 97.07 %-106.13 %. The percentage relative standard deviation for intra-day precision was 0.44 and inter-day precision was 0.59. The limit of detection was 1.1 µg/ml and limit of quantitation was 3.33 µg/ml respectively. The method has shown good and consistent recoveries and is validated as per International Council for Harmonisation guidelines and can be used for routine quality control analysis of evogliptin in dosage form.

Keywords

Dipeptidyl peptidase-4 inhibitor, ultraviolet spectrophotometric method, method development, validation, percentage relative standard deviation

Evogliptin is an anti-diabetic drug, which comes under the class of gliptin derivatives for the inhibition of selective Dipeptidyl Peptidase-4 (DPP-4) inhibitor. It was first approved in South Korea for treating the patient of type 2 diabetes for lowering of blood glucose [1]. It was taken orally by the dose of 5 mg once daily without regard to food [2]. In the structure of evogliptin, it contains a small molecule of piperazine derivative that potently inhibits DPP-4 with high selectivity. DPP-4 is the enzyme responsible for rapidly degrading the incretin hormones Glucose-dependent Insulinotropic Polypeptide (GIP) and Glucagon-Like-Peptide (GLP-1). GIP and GLP-1 are the two primary incretin hormones secreted from K and L cells, respectively [3]. In gastrointestinal tract, its response to the ingestion of nutrients, they stimulate insulin secretion from pancreatic β-cells. GLP-1, which also suppresses glucagon secretion, promotes hepatic glycogen storage and slows gastric emptying, among other actions. Thus, inhibition of DPP-4 increases the plasma levels of GLP-1, promotes insulin secretion and reduces blood glucose levels. International Union of Pure and Applied Chemistry name of evogliptin is (3R)-4- [(3R)-3-amino-4-(2,4,5-triflurophenyl)butanoyl]-3- [(tert-butoxy)methyl]piperazin-2-one, which have the chemical formula of C19H26F3N3O3 and molecular weight of 401.43 (fig. 1) [4-8].

IJPS-evogliptin

Fig 1: Chemical structure of evogliptin

While reviewing of literature for analytical method of validation it was observed that till now there are no such literature found about the method validation of evogliptin. Hence the present research work is based on the analytical method development and validation of evogliptin in bulk dosage form by Ultraviolet (UV)-spectrophotometric method. The objective of this research is to develop and validate the analytical method validation of evogliptin in bulk dosage form by UV-spectrophotometry by studying different parameters as per International Council for Harmonisation (ICH) Q2R1 guidelines.

Materials and Methods

Instrumentation and chemicals:

The instrument employed in this research work is UV-visible spectrophotometer made by Shimadzu (UV-1800), evogliptin (Alkem laboratories, India), distilled water. On the basis of their solubility study, water is selected as a solvent system.

Determination of wavelength of maximum absorption (λmax) [9-11]:

A standard stock solution of evogliptin was prepared using water as solvent. Then, the reference solution was scanned in the wavelength region of 190-400 nm.

Preparation of standard stock solution:

1 mg of evogliptin was weighed out and transferred in a 10 ml volumetric flask, dissolved with water and made up the volume with the same. Then 1 ml was taken out from this solution and again diluted up to 10 ml with the same solvent to get 10 µg/ml solution of evogliptin standard.

Preparation of standard sample solution:

Average weight of 10 marketed evogliptin tablets was taken and recorded. Weight equivalent to 1 mg of evogliptin tablet was taken and mixed with water in a 10 ml volumetric flask and made the volume up to the mark with the same solvent and filter it. Then 1 ml of filtrate solution was taken out from this solution and diluted upto 10 ml with the same solvent in a 10 ml volumetric flask to get a concentration of 10 µg/ml. Both the solution was taken to UV-spectrophotometer for further analysis.

Method validation parameters [12]:

Linearity and range: Six solutions of different concentration were prepared from the standard stock solution of evogliptin for linearity study. The absorbance of these solutions was observed and water as blank at 266 nm and the obtained data was used for linearity calibration curve.

Accuracy: Accuracy of the developed method was carried out by performing recovery studies using standard addition method, in which standard drug was added in three different concentrations (50 %, 75 % and 100 %) to the pre-analysed formulation (10 µg/ml). The result of recovery studies is expressed in percentage Relative Standard Deviation (% RSD).

Precision: Precision of the method was performed by intra-day and inter-day variation studies. The intra-day precision and inter-day precision was ascertained by determining absorbance of six replicates of the fixed concentration of the drug 10 µg/ml at six different time period of the same day and on six different days. The result of precision studies is expressed in % RSD.

Limit of Detection (LOD) and Limit of Quantitation (LOQ): The LOD and LOQ was determined with the help of linearity curve, for the assay was calculated using the following formula.

LOD=3.3×(standard deviation of y-intercept of the regression line/slope of the calibration curve)

LOQ=10×(standard deviation of y-intercept of the regression line/slope of the calibration curve)

Robustness and Ruggedness: Robustness of the method was determined by measuring the absorbance of 10 µg/ml solution of evogliptin at 263 nm, 266 nm and 269 nm. Ruggedness of the method was determined on carrying out the method by two different analysts.

Results and Discussion

During the method developmental stage, the drugs were scanned in the UV range of 190-400 nm to observe their respective λmax i.e., the wavelength at which the drug shows maximum absorbance in their respective spectra. From the spectra, evogliptin showed absorbance maxima at 266 nm (fig. 2).

IJPS-standard

Fig 2: Spectrum of standard evogliptin

Evogliptin showed linearity over the range of 2-48 µg/ml. The correlation coefficient value obtained was 0.996 with the regression equation y=0.0032x+0.005 (Table 1 and fig. 3). Inter-day and Intra-day precision studies were carried out by taking six replicates sample. Values of percentage RSD for intra-day precision were 0.44. Similarly, for inter-day precision percentage RSD was found to be 0.59 (Table 2 and Table 3).

IJPS-linearity

Fig 3: Linearity plot of evogliptin

Parameters Evogliptin
Linearity 2-48 µg/ml
Slope 0.003
Intercept 0.005
Correlation coefficient 0.996

Table 1: Regression and Characteristics of Evogliptin

Parameters Evogliptin
% purity 100.16 %
100.51 %
100.47 %
101.20 %
100.83 %
101.33 %
Mean 100.75 %
Standard deviation 0.45 %
RSD 0.44 %

Table 2: Intra-Day Precision Data

Recovery study was performed by standard spiking method, with view to justify the accuracy of proposed method. The experiment was performed in triplicate percentage recovery, mean percentage recovery was calculated for each concentration. The method has good and consistent recoveries ranging from 97.07 %-106.13 % (Table 4). Robustness was determined by increasing and decreasing the λmax of the sample and the experiment was performed in triplicate and percentage RSD was calculated for each λmax (Table 5). Ruggedness was determined by estimation of drug by different analyst using same procedure in different days and the experiment was performed in triplicate and the percentage RSD shows the good ruggedness of the given method (Table 6). LOD and LOQ was calculated using formula and calibration curve and the standard deviation was calculated by the response of blank. The LOD was 1.1 µg/ml and LOQ was 3.3 µg/ml respectively (Table 7).

Parameters Evogliptin
% purity 101.43 %
99.87 %
100.11 %
101.40 %
101.37 %
100.77 %
Mean 100.95 %
Standard deviation 0.60 %
RSD 0.59 %

Table 3: Inter-Day Precision

Drug Concentration of sample (µg/ml) Concentration of standard added (µg/ml) Recovery % Mean % recovery (n=3)
Evogliptin 10 5 100.0 99.98
89.47
110.52
10 7.5 101.75 97.07
94.73
94.73
10 10 107.89 106.13
102.63
      107.89  

Table 4: Accuracy Data

Wavelength 263 nm 266 nm 269 nm
Mean (% purity) 105.23 97.28 102.27
Standard deviation 1.03 1.58 1.57
% RSD 0.97 1.62 1.53

Table 5: Robustness Data

Parameter Analyst I Analyst II
Mean 99.99 % 99.95
Standard deviation 2.52 0.6
% RSD 2.52 0.6

Table 6: Ruggedness Data of both the Analyst

Parameters Evogliptin
Slope 0.003
Standard deviation of response 0.001
LOD 1.1 µg/ml
LOQ 3.33 µg/ml

Table 7: LOD and LOQ Data

After the literature survey, there are no previous works on the analytical method validation of evogliptin in pharmaceutical dosage form by UV-spectrometric method, so this method is developed in according to the ICH Q2R1 guidelines. In the present work evogliptin showed good solubility in water, the spectral analysis showed the λmax of evogliptin as 266 nm when water as the solvent system. The calibration curve or linearity was obtained for series of concentration range of 2-48 µg/ml and a linear relationship response was obtained when the absorbance was plot against concentration, the calibration curve for evogliptin drug was found to be linear and hence suitable for estimation of the drug. Based on their linearity or standard curve, the assay concentration was chosen as 10 µg/ml [13]. The proposed method was validated for linearity, range, accuracy, precision, robustness, ruggedness, LOD and LOQ as per ICH guidelines. The accuracy was measured in spiking method which was determined by three different concentration like 50 %, 75 % and 100 % and the method has good and consistent recoveries ranging from 97.07 %-106.13 %, which shows that the percentage recoveries of evogliptin is good as compare to the other class of gliptin derivatives. The precision was measured in repeatably like intra-day and inter-day precision. The percentage RSD for evogliptin were 0.44 and 0.59 for intra-day and inter-day precision respectively, which was performed under the lab and in different days and different times, the precision results which are in the acceptable limit and as the less value suggest that the method is precise. The results of robustness were performed in triplicate sample in some variation or different wavelength and it shows as good % RSD value and it suggest that the sample can be detected in the upper and lower range of the wavelength of evogliptin and as the value of percentage is less than 2 so the method is robust. Ruggedness was found to be 2.54 and 0.60 for the analyst I and analyst II, the robustness result shows that the changes of analyst which changes the assay result. The LOD was 1.1 µg/ml and LOQ was 3.33 µg/ml respectively, as the result shows that the instrument can detect and quantified only at this amount, which shows that the method is good and reliable. All these results show that the percentage purity or assay value of the Evogliptin in pharmaceutical dosage with its label claim shows that its good assessment of the product. The method was validated and according to the other class of gliptin derivatives because no one has been working previously on this drug, so this was validated [14]. Hence the analytical method for evogliptin in pharmaceutical dosage form was validated as per ICH guidelines [15].

In conclusion, the objective of the present work was to develop and validate the analytical method validation of evogliptin in bulk dosage form by UV-spectrophotometry in different parameter and to validate the method as per ICH guidelines. The developed UV spectrophotometric method was simple, sensitive, accurate, precise and economic and cost of materials and labours. The method has shown good and consistent recoveries and is validated as per ICH guidelines and can be used for routine quality control analysis of evogliptin in dosage form.

Acknowledgements:

Authors are grateful to Department of Pharmaceutical Analysis and Quality Assurance, Himalayan Pharmacy Institute, Sikkim for providing the facility to carry out the work on UV-spectrophotometry is highly acknowledged. Author would like to thank Dr. Biswajit Dash for guiding me for the research work.

Conflict of interest:

The authors declare no conflict of interest in this work.

References