A new high performance liquid chromatographic method for simultaneous determination of 1-and 2-naphthols in urine.
Dept. of Pharm. Chemistry Faculty of Pharmacy,
O E Izedonmwen Dept. of Pharm. Chemistry Faculty of Pharmacy
A senstive, specific and rapid HPLC procedure has been developed for the detection and determination of urine levels of 1- and 2-naphthols. Urine samples were adjusted to pH 1.5 and extracted with dichloromethane. Separation on a HPLC micropak CN-10 column with a fixed wavelength U.V. detector (254 nm) and hexane : dichloromethane : isopropylalcohol (89 : 10 : 1) as solvent system allowed the measurement of 1-naphthol at 100 ng ml(-1) and 2-naphthol at 50 ng ml(-1) levels.