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Abstract

An Improved HPLC Method Of Analysis Of Rofecoxib

Author(s): J. K Lalla, P. D Hamrapurkar, S. P Yadav, P. M Vyas

An improved, simple, specific and more sensitive method for quantitative determination of rofecoxib from the human plasma is described. The method consists of pre-column photoactivation of rofecoxib in acetonitrile by exposure to UV light at 366 nm. The drug probably undergoes a stilbene-phenanthrene like photocyclization reaction with resulting formation of a highly fluorescent species. Subsequent injection on C18e column in HPLC with fluorescent detector at 260 nm excitation and emission at 381 nm with a mobile phase consisting of water:acetonitrile (55:45 v/v) could determine rofecoxib quantitatively in the concentration range of 10 to 350 ng/ml using 20 μl of samples. The assay has been validated and has been successfully utilized in clinical studies.