Antioxidant Activity of the Aqueous Extract of Iris taochia and Identification of its Chemical Constituents
Ataturk University, Science Faculty, Molecular Biology and Genetics Department, 1Ataturk University, Pharmacy Faculty, Analytical Chemistry Department, 2Ataturk University, Science Faculty, Chemistry Department, 3Amasya University, Education Faculty, Primary Education Department, 05100-Amasya/Turkey
Ataturk University, Science Faculty, Molecular Biology and Genetics Department, Turkey, E-mail: [email protected]
In this study, phytochemical components and antioxidant capacity of extracts obtained from Iris taochia plant, which is endemic to Caucasus, were investigated. Fifteen new compounds were detected and identified using a GC-MS Sytem, which were isolated from Iris taochia for the first time. From the calibration curve, the amount of α-amyrin in dry powder of Iris taochia was calculated. The mean amount of α-amyrin found in whole plant powder of Iris taochia was 4.598 μg/10 mg with a percent recovery of 0.045 % for α-amyrin. Antioxidant properties of Iris taochia, in vitro using Fe3+-Fe2+, Cu2+-Cu+ and Fe3+-2,4,6-tripyridyl-s-triazine reducing, 1,1-diphenyl-2-picrylhydrazyl scavenging, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) scavenging, N,N-dimethyl-p-phenylenediamine scavenging and Fe2+ chelating activities were measured. The antioxidative effects of Iris taochia have not been reported earlier. These results shown that crude ethanol and water extracts of Iris taochia had effective reducing effect, radical scavenging activity and metal chelating effects when compared to standard antioxidants. The results indicated that the ethanol and water extracts of Iris taochia could be used as a potential source of natural antioxidants by the pharmaceutical industry or as a conceivable food supplement.