Comparative Evaluation Of Different Bacterial Media For Total Aerobic Bacterial Count In Deionised Water
Deionised water is used in the production of active pharmaceutical ingredients and pharmaceutical applications such as cleaning of equipments. It must meet the prescribed chemical and microbiological requirements. Total aerobic bacterial count is estimated to evaluate the microbiological quality of deionised water. Deionised water samples were tested for total aerobic bacterial count on three different media, plate count agar, soyabean casein digest agar and R2A agar using pour plate method. The results obtained were compared and it was observed that R2A agar gave slightly higher results as compared to plate count agar media while the count on soyabean casein digest agar were significantly low. Therefore, on the basis of the comparative validation of media, it can be decided whether low nutrient media like R2A agar is to be included or excluded in the routine testing, thus saving resources.