Cytotoxicity Studies of the Chemical Constituents from Marine Algae Chara baltica
K L College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram-522 502, 1Shri Vishnu College of Pharmacy, Bhimavarm-534 202, 2Anacipher Clinical Research Organization, Hyderabad-500 013, 3Government Polytechnic College, Madhavadhara, Visakhapatnam-530 007, 4Srikrupa Institute of Pharmaceutical Sciences, Velikatta, Kondapak, Siddipet-502 277, India
K L College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram-522 502, India, Email: [email protected]
For the first time, 89 chemical constituents were identified by gas chromatography-mass spectrometry analysis of ethanol extract of Clathria baltica. The chemical examination of ethanol extract of Clathria baltica yielded three known metabolites, 1, 2 and 3, for first time from the marine algae Clathria baltica. All three metabolites and the ethanol extract of Clathria baltica were screened against cancer cell lines, MCF-7, DLD-1, HeLa, FADU, A549, and SKOV3, and one normal human cell line using sulforhodamine B assay and doxorubicin as the standard. Among all isolates, compound 3 showed significant growth inhibition of MCF-7, DLD-1 and FADU with IC50 values of 26.5, 15.5 and 16.5 µg/ml, respectively. Whereas, all the metabolites and the extract exerted lower growth inhibitory effect on the normal human cell line tested. This is the first in vitro gas chromatography-mass spectrometry analysis, as well as, cytotoxicity report on marine algae Clathria baltica.