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Abstract
Determination of ebastine in pharmaceutical formulations by HPLC
Author(s): SL Prabu1, C Dinesh Kumar2, A Shirwaikar3, Annie Shirwaikar2
1Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal-576 104, India 2Department of Pharmacognosy,Manipal College of Pharmaceutical Sciences, Manipal-576 104, India 3Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal-576 104, India
Correspondence Address:
A Shirwaikar Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal-576 104 India E-mail: arunshirwaikar@yahoo.co.in
1Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal-576 104, India 2Department of Pharmacognosy,Manipal College of Pharmaceutical Sciences, Manipal-576 104, India 3Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal-576 104, India
Correspondence Address:
A Shirwaikar Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal-576 104 India E-mail: arunshirwaikar@yahoo.co.in
A simple, precise and rapid RP-HPLC method was developed for the determination of ebastine in pharmaceutical formulations. The method was carried out on a Phenomenex RP-C18 column using a mixture of methanol and water (90:10) and detection was done at 262 nm. The linearity range was 5-100 μg/ml. The intra-day and inter-day precision were in the range of 0.22% to 0.49% and 0.24% to 0.73%, respectively.
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