Development And Validation Of a HPTLC Method For The Simultaneous Estimation Of Cefuroxime Axetil And Probenecid
A simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed and validated for the determination of cefuroxime axetil and probenecid simultaneously in combined dosage form. The stationary phase used was precoated silica gel 60F254. The mobile phase used was a mixture of chloroform: acetonitrile: toluene: acetate buffer of pH 6 (5:4:1:0.3 v/v). Detection of spots was carried out at 266 nm. The method was validated in terms of linearity, accuracy, precision and specificity. The limit of detection and the limit of quantification for the drug combination were found to be 50 ng/spot and 100 ng/spot, respectively. The proposed method can be successfully used to determine the drug content of marketed formulation.