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Abstract

Estimation Of Cyclopentolate Hydrochloride From Ophthalmic Solutions

Author(s): K. V Kanna Rao, M. N Reddy, S. S Rao, M. E. B Rao

One visible spectrophotometric and another fluorimetric method have been developed for the determination of cyclopentolate hydrochloride from bulk and ophthalmic solutions. Spectrophotometric method is based on the formation of greenish blue colored species on treatment with Folin-Ciocalteu (FC) reagent in alkaline medium, showing maximum absorbance at 733 nm that obeyed Beer's law in the concentration range of 20-240 µg/ml. Fluorimetric method is based on the hydrolysate solution of cyclopentolate hydrochloride in 0.5 N sodium hydroxide on excitation at 366 nm emits yellowish blue fluorescence measured at 475 nm, which showed linearity in the concentration range of 20-600 µg/ml.