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Abstract

Estimation Of Scopoletin In Leaf And Leaf Callus Of Convolvulus Microphyllus Sieb.

Author(s): R Zafar, Syeed Ahmad, Mohd Mujeeb

The leaf callus of Convolvulus microphyllus was initiated and developed in Murashige and Skoog medium supplemented with 2,4-dichlorophenoxyacetic acid, 6-benzyl adenine, indole acetic acid and kinetin (1 ppm each). High pressure liquid chromatographic method using UV detector has been developed for the estimation of scopoletin in methanolic extracts of leaf and its calli. The method is simple, precise, accurate, specific and less time consuming for the estimation of scopoletin in crude drugs.