HPTLC analysis of hepatoprotective diterpenoid andrographolide from Andrographis paniculata nees (kalmegh)
Germplasm Evaluation Division, National Bureau of Plant Genetic Resources, New Delhi - 110 012, India
Archana P Raina Germplasm Evaluation Division, National Bureau of Plant Genetic Resources, New Delhi - 110 012 India E-mail: [email protected]
A rapid, accurate and simple high performance thin layer chromatography method for quantitative estimation of andrographolide in Andrographis paniculata is described here. The assay combines separation of andrographolide on silica gel 60 F 254 HPTLC plates followed by scanning of the spots at 232 nm using a Camag Scanner 3. Thirty germplasm collections of Andrographis paniculata were evaluated for andrographolide content by this method. Andrographolide content ranged from 1.14% to 2.60%.