Isolation of liver aldehyde oxidase containing fractions from different animals and determination of kinetic parameters for benzaldehyde
Department of Pharmaceutical Chemistry, Bombay College of Pharmacy, Kalina, India
K R Iyer, Department of Pharmaceutical Chemistry, Bombay College of Pharmacy, Kalina, India, E-mail: [email protected]
Aldehyde oxidase activity containing fractions from rabbit, guinea pig, rat and mouse livers were obtained by heat treatment and ammonium sulfate precipitation. Aldehyde oxidase activity was observed in rabbit and guinea pig livers, while aldehyde oxidase activity was absent in rat and mouse liver fractions. Enzyme kinetic parameters, K m and V max , were determined for the oxidation of benzaldehyde to benzoic acid by rabbit and guinea pig liver fractions, by spectrophotometric method, with potassium ferricyanide as the electron acceptor. The K m values obtained for both animal liver fractions were in the range of 10.3-19.1 µM.