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Abstract

RP-HPLC determination of atorvastatin calcium and amlodipine besylate combination in tablets

Author(s): DA Shah1, KK Bhatt2, MB Shankar2, RS Mehta2, TR Gandhi1, SL Baldania1
1 Anand Pharmacy College, Opp. Town Hall, Anand-388 001, India 2 A. R. College of Pharmacy, P. Box No. 19, Vallabh Vidyanagar-388 120, India

Correspondence Address:
D A Shah Anand Pharmacy College, Opp. Town Hall, Anand-388 001 India E-mail: [email protected]


A simple, specific, accurate and precise reverse-phase high-performance liquid chromatographic method was developed for the simultaneous determination of atorvastatin calcium and amlodipine besylate in tablet dosage forms. A phenomenex Luna C-18, 5 µm column having 250 × 4.6 mm i.d. in isocratic mode, with mobile phase containing methanol: acetonitrile: 50 mM KH2PO4 (20:50:30; pH 3.5) was used. The flow rate was 1.0 ml/min and effluent was monitored at 240 nm. The retention time of atorvastatin calcium and amlodipine besylate was 7.6 min and 3.2 min respectively. The linearity for atorvastatin calcium and amlodipine besylate was in the range of 5-120 µg/ml and 5-100 µg/ml respectively. The proposed method is accurate, precise, specific and rapid for simultaneous estimation of atorvastatin calcium and amlodipine besylate in tablets.



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