All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

RP-HPLC estimation of venlafaxine hydrochloride in tablet dosage forms

Author(s): SL Baldania1, KK Bhatt2, RS Mehta2, DA Shah1, Tejal R Gandhi1
1 Department of Pharmaceutical Chemistry, Anand Pharmacy College, Opp. Town Hall, Anand - 388 001, India 2 Department of Pharmaceutical Chemistry, A. R. College of Pharmacy, P.Box No. 19, Vallabh Vidyanagar - 388 120, India

Correspondence Address:
S L Baldania, Department of Pharmaceutical Chemistry, Anand Pharmacy College, India, E-mail: [email protected]


A simple, specific, accurate, and precise reverse phase high performance liquid chromatographic method was developed and validated for the estimation of venlafaxine hydrochloride in tablet dosage forms. A Phenomenex Gemini C-18, 5 µm column having 250 x 4.6 mm i.d. in isocratic mode, with mobile phase containing methanol: 0.05 M potassium dihydrogen orthophosphate (70:30, v/v; pH 6.2) was used. The flow rate was 1.0 ml/min and effluents were monitored at 226 nm. Carbamazepine was used as an internal standard. The retention time of venlafaxine hydrochloride and carbamazepine were 3.7 min and 5.3 min, respectively. The method was validated for specificity, linearity, accuracy, precision, limit of quantification, limit of detection, robustness and solution stability. Limit of detection and limit of quantification for estimation of venlafaxine hydrochloride were found to be 100 ng/ml and 300 ng/ml, respectively. Recoveries of venlafaxine hydrochloride in tablet formulations were found to be in the range of 99.02-101.68%. Proposed method was successfully applied for the quantitative determination of venlafaxine hydrochloride in tablet dosage forms.



Share this