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Abstract

Rutin-mediated Apoptosis and Glutathione Changes in Ascites Daltons Lymphoma Cells: In silico Analysis of Rutin Interactions with Some Antiapoptotic and Glutathione-related Proteins

Author(s): R. Prasad, S. Banerjee1, C. E. Kharshiing1, A. Bhattacharjee1 and S. B. Prasad*
Cell and Tumor Biology Laboratory, Department of Zoology, 1Computational Biology Laboratory, Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong-793 022, India

Correspondence Address:
Cell and Tumor Biology Laboratory, Department of Zoology, North-Eastern Hill University, Shillong-793 022, India, E-mail: [email protected]

Rutin-induced changes in apoptosis and reduced glutathione levels was studied in murine ascites Dalton’s lymphoma cells under different treatment conditions followed by the binding affinity of rutin and cis-diamminedichloroplatinum (II) with two antiapoptotic proteins and two glutathione-related enzymes was examined through molecular docking analysis. Rutin, as well as cis-diamminedichloroplatinum (II) induced apoptosis and a decrease in reduced glutathione level in Dalton’s lymphoma cells. The molecular interactions of rutin, cis-diamminedichloroplatinum (II) and a known inhibitor of the protein/enzyme showed that the binding free energies (ΔG) with antiapoptotic proteins, Bcl-xL and c-FLIP, and glutathione-related enzymes such as glutathione S-transferase and glutathione reductase were closer and comparable, which might suggest inhibitory potential of rutin on these proteins/enzymes and could play a role in its anticancer activity.