All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

Simple, Precise and Accurate HPLC Method of Analysis for Nevirapine Suspension from Human Plasma

Author(s): S Halde, A Mungantiwar, M Chintamaneni

A selective and sensitive high performance liquid chromatography with UV detector (HPLC-UV) method was developed and validated from human plasma. Nevirapine and internal standard (IS) zidovudine were extracted from human plasma by liquid-liquid extraction process using methyl tert-butyl ether. The samples were analysed using Inertsil ODS 3, 250×4.6 mm, 5 μ column using a mobile phase consists of 50 mM sodium acetate buffer solution (pH-4.00±0.05): acetonitrile (73:27 v/v). The method was validated over a concentration range of 50.00 ng/ml to 3998.96 ng/ml. The method was successfully applied to bioequivalence study of 10 ml single dose nevirapine oral suspension 50 mg/5 ml in healthy male volunteers.



Share this