Simultaneous Determination Of Cefazolin And Cefotaxime From Injections By Reverse Phase HPLC
A new simple, precise, rapid and selective reverse phase high performance liquid chromatographic method is developed for the simultaneous determination of cefazolin and cefotaxime in injections. Microbondapak C-18 30 cm column is used as a stationary phase and 85:15:1.46 ml/L (Water:Acetonitrile:H3PO4) v/v and adjusted to pH 3.5 with triethylamine is used as a mobile phase. The flow rate is 1.5 ml/min and effluent is monitored at 254 nm. The method is linear in the concentration range of (40-200) mcg/ml for cefazolin and cefotaxime respectively.