Simultaneous estimation of piperacillin and tazobactam in injection formulations
1 Department of Quality Assurance, Al-Ameen College of Pharmacy, Bangalore-560 027, India 2 Department of Pharm. Chemistry, Al-Ameen College of Pharmacy, Bangalore-560 027, India
PNS Pai Department of Quality Assurance, Al-Ameen College of Pharmacy, Bangalore-560 027 India E-mail: [email protected]
An high performance liquid chromatography method for simultaneous estimation of piperacillin and tazobactam was developed using Wakosil II, C18, 250 × 4.6 mm, 5 µm column, with mobile phase composition of methanol, phosphate buffer-pH 4 and acetonitrile in the ratio of 1:2:1 v/v/v with the flow rate of 1 ml/min and UV detection at 220 nm. The retention time for piperacillin and tazobactam was found to be 6.4 and 3.1 min respectively. Linearity was observed over the concentration range of 10-80 µg/ml for piperacillin and 2-10 µg/ml for tazobactam. Recovery was found to be 100.7-104.7% for piperacillin and 103.6-105.7% for tazobactam.