Spray-dried Ketoprofen Lysine-incorporated PLGA Nanoparticles; Formulation, Characterization, Evaluation and Cytotoxic Profile
Anadolu University, Graduate School of Health Sciences, 1Anadolu University, Faculty of Pharmacy, Department of Pharmaceutical Technology, 2Anadolu University, Faculty of Pharmacy, Department of Pharmaceutical Biotechnology, Eskişehir, Turkey
Anadolu University, Faculty of Pharmacy, Department of Pharmaceutical Technology, Eskişehir, Turkey, E-mail: [email protected]
Ketoprofen lysine because of a short half-life (1-2 h) multiple dosing regimens are required for oral administration. For this reason, this study is concerned with the preparation of poly lactic-co-glycolic acid-based spray-dried nanoparticles to extend the activity of the ketoprofen lysine. Nanoparticles were produced with a spray dryer (B-90, Büchi, Switzerland) and solid state properties of nanoparticles were analyzed using scanning electron microscopy, particle size, polydispersity index, zeta potential, nuclear magnetic resonance spectroscopy, X-ray and differential scanning calorimetry. Drug release from nanoparticles was studied using the dialysis bag method in simulated gastrointestinal environment (pH 7.4). Ketoprofen lysine-loaded particles demonstrated nanostructured and spherical shape while in vitro release studies showed extended release (~8 days) of ketoprofen lysine incorporated and also a fairly good entrapment efficiency (~59-78%) was detected. Peppas-Sahlin kinetic model was found to fit the best using DDSolver software program. This research may be useful for long-term and optimum use of oral non-steroidal antiinflammatory drug in chronic inflammatory diseases.