Tissues and Plasma Drug Concentrations Following Intraperitoneal Administration of Amphotericin B Prepared in Acetate/Acetic Acid Buffer Solution
Laboratory Antifungals Antibiotics, Physicochemical, Synthesis and Biological Activity (LAPSAB), Department of Biology, University of Tlemcen, Tlemcen 13000, University Center of Aïn Temouchent, 46000, Department of Biology, University of Ghardaia, Ghardaia 47000, Algeria
Laboratory Antifungals Antibiotics, Physicochemical, Synthesis and Biological Activity (LAPSAB), Department of Biology, University of Tlemcen, Tlemcen 13000, Algeria E-mail: [email protected]
The aim of this work is to study the effect of the physicochemical environment on in vivo distribution of amphotericin B, which was prepared in acetic acid/acetate buffer pH 5.4 at a concentration of 10-4 M. Rats were injected intraperitoneally with this antifungal solution at the doses of 1, 2 or 5 mg/kg body weight. At predefined time, amphotericin B from tissues and plasma was extracted and analysed using the second derivative spectrophotometry methodology. The level of amphotericin B in tissues and plasma depended on the administered dose and decreased over a period of time. The highest concentrations were found in the spleen, followed by the liver, but were lower in kidneys and plasma. Amphotericin B was accumulated in the intraperitoneal cavity (the peritoneal adipose tissue) in large quantities proportional to the dose administered intraperitoneally. This formulation has the advantage of being easy to prepare and inexpensive, it seems very promising for the treatment of fungal infections of spleen and peritoneal cavity.