*Corresponding Author:
H. C. Andola
Centre for Aromatic Plants (CAP), Industrial Estate, Selaqui-248 197
E-mail: [email protected]
Date of Submission February 06, 2012
Date of Revision May 20, 2012
Date of Acceptance June 01, 2012
Indian J Pharm Sci 2012 74 (3): 265-267  

Abstract

The essential oils isolated from the aerial parts of two different populations of Artemisia gmelinii growing in Uttarakhand Himalaya region were analysed by gas chromatography and gas chromatography/mass spectrometry (GC-MS) in order to determine the variation of concentration in their constituents. Artemisia ketone was detected as a major constituent in both the populations i.e., Niti valley and Jhelum samples. Niti oil was found to have considerably greater amounts of artemesia ketone (53.34%) followed by α-thujone (9.91%) and 1,8-cineole (6.57%), Similarly, the first major compound in Jhelum oil was artemesia ketone (40.87%), whereas ar-curcumene (8.54%) was identified as a second major compound followed by α-thujone (4.04%). Artemisia ketone can be useful for perfumery and fragrance to introduce new and interesting herbaceous notes.

Keywords

Artemesia ketone, Artemisia gmelinii, Asteraceae, essential oil composition

Artemisia gmelinii Weber ex Stechm. (Asteraceae), a bushy aromatic shrub, is found in the alpine regions of Kashmir, Kinnaur and Kumaon on the interior ranges bordering Tibet between altitudes of 2700-5100 m [1,2]. In Uttarakhand Himalaya, the herb of A. gmelinii is used as incense by the local inhabitants due to its aromatic odour. The essential oil of Artemisia species is also finding its place in the indigenous perfumery industries. The essential oil composition of different Artemisia species viz. A. capillaris, A. edgeworthii, A. gmelinii, A. maritima, A. myriantha, A. nilagirica, A. parviflora, A. roxburghiana, A. verlotiorum and A. wallichiana, has been a matter of investigation for several investigators in India, especially from Uttarakhand region. Major compounds are thujone, b-caryophyllene, b-eusesmol, g-terpinene, 1,8-cineole, ar-curcumene, artemisia ketone, borneol, camphor, capillene, caryophyllene oxide, chrysanthenone, germacerene D, terpinen-4-ol and vulgarone B have been reported [3-13].

In spite of many essential oil related studies on the genus Artemisia, there are still many problems in systematic interpretations. The quality and quantity of the essential oil varies a lot with the genetic makeup of the taxa and the prevalent environmental factors. In the present study, both the oils were dominated by artemisia ketone which has green herbaceous odour and has been suggested for use in perfumery to introduce new and interesting herbaceous notes, particularly for men’s fragrances [14]. In view of the above, the present study is designed so as to study the quantitative and qualitative variation in the constituents of A. gmelinii.

The aerial parts of A. gmelinii were collected from Niti valley (3620 m) and Jhelum (3000 m) regions of Uttarakhand Himalaya in the month of September, 2007. Voucher specimen has been duly identified and deposited in the herbarium of Botanical Survey of India (BIS), northern circle, Dehradun (BSD 112167).

Air dried aerial parts (300 g) of the plants were hydro-distilled for 3 h using a Clevenger-type apparatus. The oils were greenish in colour and had characteristic odour. The distillated oils were dried over anhydrous sodium sulphate, and stored in tightly closed vials at 4° for analysis. The essential oil content was determined as percentage on fresh weight basis as an average of three independent extractions of each site to minimise error. The combined oil was used for further analysis.

About 0.1 μl of each pure oil sample was subjected to gas chromatography (GC) and gas chromatography/ mass spectrometry (GC/MS) analyses. The GC was composed of an Agilent Technology 6890 N gas chromatograph data handling system equipped with a split-splitless injector and fitted with a flame ionization detector (FID) using N2 as the carrier gas. The column was HP-5 capillary column (30 m×0.32 mm, 0.25 μm film thickness) and temperature program was used as follows: Initial temperature of 60° (hold: 2 min) programmed at a rate of 3°/min to a final temperature of 220° (hold: 5 min). Temperatures of the injector and FID were maintained at 210° and 250°, respectively.

The GC/MS analyses were carried out on a Perkin Elmer Clarus 500 gas chromatograph equipped with a split-splitless injector (split ratio 50:1) data handling system. The column was an Rtx®-5 capillary columns (60 m×0.32 mm, 0.25 μm film thickness). Helium (He) was the carrier gas at a flow rate 1.0 ml/min. The GC was interfaced with (Perkin Elmer Clarus 500) mass detector operating in the EI+ mode. The mass spectra was generally recorded over 40-500 amu which revealed the total ion current (TIC) chromatograms. Temperature program used was the same as described above for GC analyses. The temperatures of the injector, transfer line and ion source were maintained at 210°, 210° and 200°, respectively.

Identification of the individual components was made by matching their recorded mass spectra with the library (NIST/Pfleger/Wiley) provided by the instrument software, and by comparing their calculated retention indices with literature value [15,16]. Relative area percentages of the individual components were obtained from GC-FID analyses.

The composition of the essential oils obtained from A. gmelinii is presented in Table 1. Altogether, 39 essential compounds were identified by GC and GC/MS representing 90.54% (Niti oil) and 89.60% (Jhelum oil). Both oils were dominated by oxygenated monoterpenes, representing 79.65% and 58.18% in Niti and Jhelum oils, respectively. The Niti oil revealed the presence of artemesia ketone (53.24%), a-thujone (9.91%) and 1,8-cineole (6.57%). Similarly, the major compound in Jhelum oil was artemesia ketone (40.87%), followed by ar-curcumene (8.54%) and a-thujone (4.04%). Although, artemesia ketone was detected as the major compound in both the sites, it decreased with the increasing altitudes. In an earlier study on A. gmelini from Uttarakhand Himalaya [13], the major constituent of the oil showed similarity with the present investigation, but the variations occurred in the quantitative composition of both the studies, whereas a report from Central Asia differed with our study with respect to their major constituents. They reported 1,8-cineole (21-40%), camphor (10-31%) and borneol (4-17%) as the major constituents in A. gmelini oil [17]. The present study concluded that in both the sites the oil components quantitatively varied from each other, but qualitatively both oils were similar except for some minor components.

Compounds RIlit RIexp Composition (%)
Niti oil Jhelum oil
Artemisia triene 923 929 3.21 3.93
α-thujene 924 930 0.09 -
α-pinene 932 939 0.11 0.81
Camphene 946 953 - 0.53
Sabinene 969 975 0.45 0.71
1-octen-3-ol 972 982 0.31 0.71
β-pinene 974 979 0.19 0.49
Myrcene 988 991 - 0.68
α-phellandrene 1002 1003 0.17 0.37
p-cymene 1020 1025 0.31 0.30
1,8-cineole 1026 1033 6.57 3.69
Artemisia ketone 1056 1062 53.34 40.87
Trans-sabinene hydrate 1098 1098 - 1.18
Nonanal 1100 1098 0.98 0.79
α-thujone 1102 1101 9.91 4.04
β-fenchyl alcohol 1112 1113 - 0.12
β-thujone 1114 1114 1.08 0.39
Camphor 1141 1142 0.98 0.89
Sabina ketone 1154 1156 0.13 0.26
Pinocarvone 1160 1161 0.52 0.23
Terpinen-4-ol 1174 1177 1.56 1.16
α-thujenal 1181 1180 - 0.27
cuminic aldehyde 1238 1239 0.99 0.55
trans-geraniol 1249 1247 3.13 2.82
decanoic acid 1364 1364 0.15 0.21
Trans-caryophyllene 1417 1419 0.17 2.36
α-guaiene 1437 1439 - 2.72
α-humulene 1452 1455 - 0.57
β-farnesene 1454 1457 0.22 0.69
ar-curcumene 1479 1474 2.31 8.54
germacrene D 1484 1485 0.31 2.06
α-zingiberene 1493 1489 0.32 1.89
β-bisabolene 1505 1509 0.24 0.55
Caryophyllene oxide 1582 1583 0.30 0.82
Vulgarone B 1649 1651 1.58 1.76
2-pentadecanone 1697 1699 0.20 0.40
Farnesol 1722 1722 0.16 0.51
Hexadecanol 1874 1870 0.25 0.32
Eicosane 2000 2000 0.30 0.41
Monoterpene hydrocarbons     4.53 7.82
Oxygenated monoterpenes     79.65 58.18
Sesquiterpene hydrocarbons     3.57 19.38
Oxygenated sesquiterpenes     2.79 4.22
Total identified     90.54 89.60

Table 1: Composition of the essential oil Of Artemisia Gmelinii collected from two Locations of uttarakhand himalaya

References