A HPLC Determination Of Betacarotene In The Fruit Juice Of Passiflora Edulis Sims
A simple, accurate and reproducible HPLC method for the determination of betacarotene in the ripe fruit juice of Passiflora edulis Sims was developed. The method involves separation of betacarotene using an isocratic mobile phase consisting of acetonitrile, methylene chloride and methanol in the ratio of 82:12:16 at 436 nm using photo diode assay detector. The sensitivity was found to be 0.25 mg. The betacarotene content of 3.21%w/v was observed in test sample. The calibration curve was linear in the range of 0.25 mg to 2.50 mg with correlation co-efficient of 0.987998 indicating good linearity between concentration and area. The method provides good resolution and separation of betacarotene from other constituents of total carotenoids. An average recovery of 99.08% w/w shows the reliability and suitability of the method.