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Abstract

A Preliminary Study on the Mechanism of Early Lesions in the Subchondral Bone of a Rat Model of Intevertebral Disc Degeneration

Author(s): Hao Qu, Feng Wang, Zhigang Li, Dan Wang, Feng Xu, Hui Kang, Wei Huang, Heng Wu, Fan Wu and Xianhua Cai*
College of Acupuncture and Orthopedics, Hubei University of Chinese Medicine, 1Department of Orthopedics, Hubei Provincial Hospital of Integrated Chinese and Western Medicine, 2Department of Orthopedics Surgery, General Hospital of Central Theater Command, Wuhan, Hubei 430000, 3Department of Orthopedics, Characteristic Medical Center of the Chinese People???s Armed Police Force, 4Department of Spine Surgery, Jinmen NO. 2 People???s Hospital, Jingmen, Hubei 448000, China

Correspondence Address:
Xianhua Cai, Department of Orthopedics Surgery, General Hospital of Central Theater Command, Wuhan, Hubei 430000, China, E-mail: 19947615161@163.com


The objective of this study is to examine the impact of caffeic acid on triple negative breast cancer by regulating epidermal growth factor receptor/signal transduction and activator of transcription 3/protein kinase B pathway related proteins. The blank control group cells were immortalized human normal breast epithelial cell line MCF-10A, without any treatment, and were cultured routinely; the cells in the triple negative breast cancer group were human triple negative breast cancer MDA-MB-231 cells, which were routinely cultured without any treatment; in caffeic acid p-nitrophenyl ethyl ester group, human triple negative breast cancer MDA-MB-231 cells were added with caffeic acid p-nitrophenyl ethyl ester 10 μg/ml. After continue to incubate in the incubator for 48 h. The proliferation activity and the number of cell migration of caffeic acid on nitrophenylethyl ester group were lower compared to the triple negative breast cancer group. The number of cell invasion of caffeic acid on nitrophenylethyl ester group was lower in comparison to the triple negative breast cancer group, and the apoptosis rate exhibited a statistically significant increase in comparison to the triple negative breast cancer group. The levels of epidermal growth factor receptor, signal transduction and activator of transcription 3, protein kinase B, messenger ribonucleic acid, and protein were found to be significantly lower in the caffeic acid group compared to the triple negative breast cancer group. The expression levels of matrix metalloproteinase-2, matrix metalloproteinase-9 and vascular endothelial growth factor-A protein in the caffeic acid nitrophenethyl group were lower in comparison to the triple negative breast cancer group. Caffeic acid p-nitrophenyl ethyl ester can reduce the activity of triple negative breast cancer cells, inhibit the growth, migration and invasion of triple negative breast cancer cells, and induce apoptosis of colorectal cancer cells. The mechanism potentially exhibits a correlation with the regulation of the epidermal growth factor receptor/signal transduction and activator of transcription 3/protein kinase B signaling pathway.

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