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Abstract
A Sensitive High Performance Liquid Chromatography Method For Determination Of Bioequivalence Of Atenolol Formulations
Author(s): S. A Shah, I. S Rathod, C. J Shishoo, S. S Savale, M. C Satia, K. M Bhat
A sensitive and specific HPLC method with fluorescence detector has been developed for the analysis of atenolol in plasma using a reverse phase column (ODS, 250 x 4.6 mm, 10 μm). Atenolol levels were measured using its excitation and emission maxima at 276 nm and 302 nm, respectively. The method has a linearity range of 50-1000 ng/ml and recovery was found to be 95.7%. This method has been successfully used for the bioequivalence study of atenolol tablets (25 mg). Cmax, tmax and AUC0-, of the test formulation statistically does not show significant difference from the standard formulation.
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