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Abstract

A supercritical fluid chromatography/tandem mass spectrometry method for the simultaneous quantification of metformin and gliclazide in human plasma

Author(s): YK Agrawal1, PJ Gogoi2, K Manna2, HG Bhatt2, VK Jain3
1 Institute of Research & Development, Gujarat Forensic Sciences University, Gandhinagar-382 007, India 2 Institute of Pharmacy, Nirma University, S. G. Highway, Ahmedabad-382 481, India 3 Chemistry Department, School of Science, Gujarat University, Ahmedabad-380 009, India

Correspondence Address:
Y K Agrawal Institute of Research & Development, Gujarat Forensic Sciences University, Gandhinagar-382 007 India E-mail: [email protected]


Present study reports the development and validation of a simultaneous estimation of metformin and gliclazide in human plasma using supercritical fluid chromatography followed by tandem mass spectrometry. Acetonitrile:water (80:20) mixture was used as a mobile phase along with liquid CO 2 in supercritical fluid chromatography and phenformin as an internal standard. The modified plasma samples were analyzed by electro-spray ionization method in selective reaction monitoring mode in tandem mass spectrometry. Supercritical fluid chromatographic separation was performed using nucleosil C 18 containing column as a stationary phase. The separated products were identified by characteristic peaks and specific fragments peaks in tandem mass spectrometry as m/z 130 to 86 for metformin, m/z 324 to 110 for gliclazide and m/z 206 to 105 for phenformin. The present method was found linear in the concentration ranges of 6.0-3550 ng/ml and 7.5-7500 ng/ml for metformin and gliclazide, respectively. Pharmacokinetic study was performed after an oral administration of dispersible tablets containing 500 mg of metformin and 80 mg of gliclazide using same techniques.

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