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Adaptation Of Labat Test To the Assay Of Piperine, Alone And In Combination With Rifampicin, Isoniazid And Nimesulide

Author(s): E Venkata Rao, P Sudheer, S. V Ramanjaneyulu

A simple and rapid colorimetric method of assay of piperine is reported. It is an adaptation of Labat test for methylenedioxy group attached to an aromatic nucleus. The resultant blue green colour shows maximum absorbance at 656 nm and obeys Beer's law in a concentration range of 10-25 �¼g/ml. Results of analysis on different piperine samples are comparable with direct spectrophotometry and HPLC methods. The method is also suitable for assay of piperine in combination with rifampicin, isoniazid, and nimesulide.


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