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Assay Of Secnidazole In Tablet Formulations By Fluorometry And Spectrophotometry

Author(s): Sadhana J Rajpur, Kalpana G Patel

Two accurate and sensitive methods are described for the determination of secnidazole in bulk drug and tablet formulations. The methods are based on the reduction of secnidazole with zinc dust and sodium hydroxide. In method I, fluorescence of the reduced product was measured. The excitation and emission wavelengths were observed at 360 nm and 425 nm respectively. A linear relationship between relative fluorescence intensity and concentration was obtained for 1 to 10 mcg of secnidazole per ml. The reduced product was diazotized and coupled with Bratton and Marshal's reagent in method II to get a chromogen showing maximum absorbance at 502 nm.


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