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Abstract

Association between Expression of Nuclear Factor-Kappa B Signaling Pathway and Apoptosis of Renal Tubular Epithelial Cells in Rats with Calcium Oxalate Kidney Stones

Author(s): X. Zheng, S. Chen, Xiaozhi Yao and Y. Chen*
Department of Pediatrics, Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, Fuzhou, Fujian Province 350001, 1Department of Immunology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian Province 350108, China

Correspondence Address:
Y. Chen, Department of Immunology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian Province 350108, China, E-mail: ycy2728@163.com


The study aimed to explore the effect of the nuclear factor-kappa B signaling pathway on apoptosis of rat renal tubular epithelial cells caused by calcium oxalate monohydrate crystals. After being cultivated in vitro, NRK-52E cells were split into a control group, 1 mm calcium oxalate monohydrate group and 10 μM QNZ+1 mm calcium oxalate monohydrate group. The optical density value was detected via cell counting kit-8 assay, the content of lactate dehydrogenase in cell supernatant was detected via colorimetry, and the expressions of nuclear factor-kappa B signaling pathway-related proteins p65 and p50 and B cell lymphoma-2, tumor necrosis factor receptor 1 and caspase-3 were determined through Western blotting. 1 mm calcium oxalate monohydrate could significantly reduce the proliferation ability of NRK-52E cell and cause cytotoxicity. 1 mm calcium oxalate monohydrate significantly up-regulated the expressions of p65, p50, tumor necrosis factor receptor 1 and caspase-3, and significantly lowered the B cell lymphoma-2. Besides, 10 μM QNZ+1 mm calcium oxalate monohydrate obviously down-regulated the expressions of p65, p50, tumor necrosis factor receptor 1 and caspase-3, and obviously increased the B cell lymphoma. The apoptosis of rat renal tubular epithelial cells in rats with calcium oxalate kidney stones may be promoted through activating the nuclear factor-kappa B signaling pathway. Inhibiting the nuclear factor-kappa B activity can alleviate apoptosis and improve renal cell function.

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