All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

Catalase in testes and epididymidis of wistar rats fed zinc deficient diet

Author(s): S Bedwal1, S Prasad1, N Nair1, MR Saini2, RS Bedwal1
1 Cell Biology Laboratory , Department of Zoology, University of Rajasthan, Jaipur-302 004, India 2 Radiation Biology Laboratory, Department of Zoology, University of Rajasthan, Jaipur-302 004, India

Correspondence Address:
R S Bedwal Cell Biology Laboratory , Department of Zoology, University of Rajasthan, Jaipur-302 004 India [email protected]


Catalase activities have been evaluated in testes and caput and cauda epididymis of Wistar rats fed on zinc deficient diet for 2 and 4 weeks. The enzyme activity has been measured as chromic acetate formed by heating of dichromate (in acetic acid) in presence of H 2 O 2 with perchromic acid as an unstable intermediate. Observed non-significant increase in catalase activity in testes as well as in caput and cauda epididymis of 2 weeks experiments has been related to low levels of H 2 O 2 produced in two organs whereas significant (P<0.01/0.001) increase in catalase activity in 4-weeks experiments indicate for increased oxidative stress due to phagocytotic activity of Sertoli cells in testes and damaged spermatozoa in epididymis. Thus, zinc deficiency increases catalase activity in testes and epididymis.

Full-Text | PDF