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Abstract

Colorimetric Determination Of Thiamine Hydrochloride Using Alzarin Briliant Violet R

Author(s): P. S. S Prasad, K. P Rajasree, K. A Khan, M Narayana Reddy

A new colorimetric method for the determination of thiamine hydrochloride is developed. It is based on the formation of coloured ion-pair complex between thiamine hydrochloride and alizarin brilliant violet R in the aqueous phase at pH 4.5 extractable into chloroform which obeyes Beer's law in the range of 2-16 mcg/ml.

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