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Abstract

Combined thin-layer chromatography-densitometry for the quantitation of curcumin in pharmaceutical dosage forms and in serum.

Author(s): AK Dwivedi, M Raman, RK Seth, J PS Sarin
Division of Pharmaceutics, Central Drug Research Institute, LUCKNOW - 226 001,

Correspondence Address:
A K Dwivedi Division of Pharmaceutics, Central Drug Research Institute, LUCKNOW - 226 001

A new TLC Densitometric method for the quantitative estimation of a nonsteroidal anti-inflammatory agent curcumin in bulk drug samples, pharmaceutical dosage forms and in human serum has been worked out. The assay combines TLC on Silicagel 60 F254 with spot visualisation by single wavelength reflection mode, excitation 265 nm and emission UV D2 filter and densitometry. This communication describes a simple and quick TLC densitometric method for the estimation of curcumin in bulk drug samples, pharmaceutical dosage forms as well as in human serum.

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