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Combined Thin-Layer Chromatography-Densitometry Method For The Quantitative Estimation Of Major Alkaloids In Poppy Straw Samples

Author(s): M. M Gupta, R. K Verma

A simple, quick and inexpensive thin layer chromatography-densitometry method for the determination of major opium alkaloids morphine, codeine, thebaine, papaverine, and narcotine in poppy straw samples is described. The assay combines the separation of compounds in Silica gel 60 F254 thin layer plates with spot visualization by spraying plates with Dragendorff reagent and densitometry by dual wavelength absorption mode.