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Continuous Production Of 6-Amino Penicillanic Acid From Benzyl Penicillin Using Immboilized E. Coli Cells

Author(s): P Bhattacharjee, S Patil, M Tripathi, S. C Chaturvedi

The effect of the type of matrix on the bio conversion of Benzyl penicillin to 6-Amino penicillanic acid (6-APA) using immobilized E.coli cells was studied. The E. coli strains selected were MTCC 476, MTCC 448, MTCC 521 and MTCC 66. The matrices selected for entrapping these cells were polyacrylamide, calcium alginate, K-carrageenin and agarose. Bioconversion was performed with free cells as well as immobilized cells, afterwards continuous production of 6-APA was carried out using a packed bed column.


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