All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

Correlation between Methylation and Expression of Kallikrein Related Peptidase 12 Gene in Breast Cancer

Author(s): Li Na and Z. Kai*
Department of Blood Transfusion, Tianjin Hospital, Tianjin 300211, China

Correspondence Address:
Z. Kai, Department of Blood Transfusion, Tianjin Hospital, Tianjin 300211, China, E-mail: 13752590428@139.com


The aim of this study is to investigate the methylation and expression of kallikrein 12 gene in triple-negative breast cancer cells. We evaluated the methylation status of the promoter region of kallikrein 12 gene in breast cancer using bisulfite sequencing polymerase chain reaction to compare the expression of kallikrein 12 gene in MDA-MB-231 and breast cancer cells and examined the expression of kallikrein 12 messenger ribonucleic acid by real-time quantitative polymerase chain reaction. In addition, we detected the expression of kallikrein 12 protein using Western blotting. The elevated methylation of the kallikrein 12 promoter in triple-negative cancer cells led to a reduction in its protein and messenger ribonucleic acid levels. But, after the treatment with 5-aza-2'-deoxycytidine, the protein and messenger ribonucleic acid expression increased. The use of 5-aza-2'- deoxycytidine can significantly reduce the level of kallikrein 12 promoter methylation in the MDA-MB-231 cells. These findings suggest that the hypermethylation may be responsible for the reduced gene expression in these cancer cells. In this study, we introduced a new concept and methodology for the treatment and diagnosis of triple-negative breast cancer.

Full-Text | PDF

 
 
Google scholar citation report
Citations : 69022

Indian Journal of Pharmaceutical Sciences received 69022 citations as per google scholar report