All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

Curcumin Mitigates Oxygen-Glucose Deprivation/Reperfusion-Induced Neuronal Damage by Downregulating microRNA-145-5p

Author(s): Hong Liang and Ruihua Sun*
Laboratory Department, The Third People's Hospital of Gansu Province, Lanzhou 730000, 1Laboratory Department, Wuwei Traditional Chinese Medicine Hospital, Wuwei 733000, China

Correspondence Address:
Ruihua Sun, Laboratory Department, Wuwei Traditional Chinese Medicine Hospital, Wuwei 733000, China, E-mail: zldongtaiping@163.com


Curcumin can alleviate cerebral ischemia/reperfusion injury, but its mediated mechanism is still unclear. The study aimed to investigate whether curcumin mediates cerebral ischemia/reperfusion injury-impelled neuronal damage by microRNA-145-5p. An oxygen-glucose-deprivation/reperfusion model was conducted using rat hippocampal neuron HT22. Detection of catalase and superoxide dismutase activities and malondialdehyde content by a matching reagent kit was carried out to assess cellular oxidative stress. Interleukin-6, interleukin-10, and tumor necrosis factor-alpha were assessed for inflammation analysis. Relative microRNA-145-5p expression was detected by quantitative polymerase chain reaction. Oxygen-glucose-deprivation/reperfusion stimulation prompted the release of interleukin-10, interleukin-6, and tumor necrosis factor-alpha, lessened the activities of catalase and superoxide dismutase, as well as elevated the content of malondialdehyde in neurons HT22, but these alterations were undercut by curcumin in a concentration-dependent manner, suggesting that curcumin impaired oxygen-glucose-deprivation/reperfusion-prompted HT22 cell inflammation and oxidative stress. MicroRNA-145-5p silencing overtly reduced oxygen-glucose-deprivation/reperfusionprompted HT22 cell inflammation and oxidative stress. Curcumin treatment significantly decreased oxygenglucose- deprivation/reperfusion-mediated upregulation of microRNA-145-5p in neurons HT22. Moreover, in oxygen-glucose-deprivation/reperfusion-stimulated neurons HT22, the upregulation of microRNA-145-5p overexpression partly reversed cur-mediated changes regarding pro-inflammatory factors and indicators of oxidative stress. Curcumin could reduce microRNA-145-5p expression in oxygen-glucose-deprivation/ reperfusion-stimulated neurons, thus weakening neuronal inflammation and oxidative stress.

Full-Text | PDF

 
 
Google scholar citation report
Citations : 69022

Indian Journal of Pharmaceutical Sciences received 69022 citations as per google scholar report