Abstract
Curcumin Suppresses JAK2/STAT3 Pathway to Ameliorate Pancreatic Injury Induced by Sepsis in Rats
Department of Critical Care Medicine, Fujian Medical University Union Hospital, Fuzhou, Fujian 350001, 1Department of Clinical Medicine, Fujian Medical University, Fuzhou, Fujian 350005, China
Correspondence Address:
Shuaijun Zhu, Department of Critical Care Medicine, Fujian Medical University Union Hospital, Fuzhou, Fujian 350001, China, E-mail: zhushuaijun@fjmu.edu.cn
The occurrence of pancreatic injury is an important pathological change of sepsis. Curcumin was used to treat organ injury by sepsis, but its effect in pancreatic injury induced by sepsis still unclear. In this study, the animal model of pancreatic injury in sepsis was constructed via tail vein injection lipopolysaccharide into rats, and the tissues and serum of rats were collected for further investigation. The pathological changes of pancreatic injury were presented by hematoxylin and eosin staining. The concentration of inflammatory factors (interleukin-6, interleukin-1 beta and tumor necrosis factor-alpha) and oxidative stress related factors (superoxide dismutase, malondialdehyde, and reactive oxygen species) were detected by enzyme-linked immunosorbent assay kits. The terminal deoxynucleotidyl transferase dUTP nick end labeling assays and Western blot were carried out to detect apoptotic rate and apoptotic-related proteins (B-cell lymphoma 2, Bcl-2-associated X protein, caspase-3 and cleaved caspase-3). The expression level of Janus kinase 2/signal transducer and activator of transcription 3 pathway was verified by Western blot. The septic pancreatic injury rats’ model were successful constructed with obvious pathological changes and enhanced expression of inflammatory factors. Curcumin could reduce the level of inflammation, oxidative stress, and apoptosis in pancreatic tissue of septic rats. In addition, curcumin ameliorate pancreatic injury induced by sepsis by inhibiting Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway, and the inhibition effect of curcumin can be reversed by adding Janus kinase 2 activator coumermycin A1. Curcumin suppresses Janus kinase 2/signal transducer and activator of transcription 3 pathway to ameliorate pancreatic injury induced by sepsis in rats, including attenuating inflammatory reaction, oxidative stress, and apoptosis. This study was supposed to provide a new research idea for the prevention and clinical treatment of pancreatic injury in sepsis.
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