Cytochrome P450 27B1 Gene Polymorphism rs10877012 and rs4646536 in Patients with Systemic Lupus Erythematosus
Department of Tropical Diseases Division, 1Department of Rheumatology, The Second Affiliated Hospital of Hainan Medical University, Haikou, Hainan 570311, 2Department of Scientific Laboratory, Hainan Medical University, Haikou, Hainan 571199, China
Weifei Chen, Department of Rheumatology, The Second Affiliated Hospital of Hainan Medical University, Haikou, Hainan 570311, China, E-mail: email@example.com
Systemic lupus erythematosus is a severe inflammatory connective tissue disease induced by autoimmunity. The cytochrome p450 27B1 gene, activating Vitamin D precursor 25-hydroxyvitamin D into 1,25-dihydroxyvitamin D in immunity cells resulted in autoimmune disorders. This study was assessing the correlation and clinical pathological characteristics relationship between the cytochrome p450 27B1 gene polymorphism rs10877012/rs4646536 and systemic lupus erythematosus to provide a rationale for determining the role that cytochrome p450 27B1 gene polymorphism plays in systemic lupus erythematosus. In this study, we investigated the association of rs10877012 (T/G) polymorphism with systemic lupus erythematosus susceptibility in 156 patients with systemic lupus erythematosus compared to 156 healthy population, both groups came from the same Mendelian population by conformity to Hardy Weinberg equilibrium. DNA was extracted from peripheral leukocytes and analyzed for the polymorphism using the polymerase chain reaction-ligase detection reaction method. We found an association between the presence of the T allele at the polymorphic site (odds ratio=2.94; 95 % confidence interval 1.77–4.86; <0.0001) and a decreased colorectal cancer incidence. rs10877012 polymorphism was associated with arthritis and positive Anti-double stranded DNA antibody and increased C-reactive protein happened in patients with systemic lupus erythematosus.