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Determination of ethanol in Abhayarishta by gas chromatography

Author(s): SJ Wadher, Manisha Puranik, PG Yeole, CS Lokhande
Institute of Pharmaceutical Education and Research, Borgaon (Meghe), Wardha-442 001, India

Correspondence Address:
S J Wadher Institute of Pharmaceutical Education and Research, Borgaon (Meghe), Wardha-442 001 India E-mail: [email protected]

A simple, rapid, precise and accurate gas liquid chromatographic method was developed for the determination of ethanol in marketed preparation of abhayarishta using flame ionization detector. The separation was performed employing carbowax 20 M stainless steel column using nitrogen as a carrier gas at optimized conditions. The column was maintained at 90°, while injection port and detector were maintained at 110°. The system suitability parameters were studied throughout the study. The method has been applied successfully for the assay of ethanol in abhayarishta . The recovery values were found to be in the range of 98.14-104.54% with RSD values less then 0.800%. The proposed method can be used for the determination of generated ethanol content in abhayarishta preparation.

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