Development and Evaluation of an Acute Asthma Model Induced by Ovalbumin and Formaldehyde Exposure in Murine
Department of Pharmaceutical Sciences and Technology, Birla Institute of Technology, Mesra-835215, Ranchi, India
P. M. Mazumder, Department of Pharmaceutical Sciences and Technology, Birla Institute of Technology, Mesra-835215, Ranchi, India, E-mail: firstname.lastname@example.org
Asthma is a common prolonged inflammatory disease of the upper respiratory tract, affecting millions of people in all age groups. At present, the treatment of asthma has become a challenge for practitioners because of the regular increment in air pollution worldwide. Subsequently, during the new drug development process, preclinical assessment of active compounds through a quick and robust model is urgently required. Thus, this study aimed to establish an acute asthma model using ovalbumin and formaldehyde in mice. In this experimental study, all animals were sensitized with ovalbumin on 0th and 5th d and formaldehyde on the 3rd, 4th, 6th, and 7th d followed by an ovalbumin challenge from 8th to 10th d. During induction of disease, individual groups received beclomethasone and rutin as standard and test drugs, respectively. At the end of the study, the whole lung was harvested, weighed, and assessed for lung weight index. Also, oxidative stress and inflammatory biomarkers were estimated, and lung histopathology was performed. In the results, it was observed that the lung weight index, total leukocyte count and differential leukocyte count, and malondialdehyde values, significantly (p<0.05) decreased, and superoxide dismutase, glutathione and catalase activity were markedly (p<0.05) increased in the rutin treated group as compared to the diseased group. Histopathological studies of lung tissue in the diseased group showed extensive perivascular edema. However, in the rutin-treated group, mucus gland hyperplasia was significantly decreased. Therefore, in this experimental research, an attempt was made to develop a successful acute asthma model within a short period, which reversed on repeated dosing with rutin in murine.