Abstract

Tuberculosis is the second most common cause of death from infectious disease. Current antituberculosis treatments consist of long course of combination of antibiotics and toxic side effects and lead to poor patient compliance. So, the present research work has been undertaken for the development and standardization of polyherbal formulation for tuberculosis. In the present study polyherbal formulation was developed using powder of plants of Ocimum sanctum Linn, Tinospora cordifolia (Willd.) Miers, Alstonia scholaris (L.) R.Br and Trikatu churna. The phytochemical screening was performed for presence of various phytoconstituents. Thin Layer Chromatography densitometric methods were developed using High-Performance Thin-Layer Chromatography for the quantifications of gallic acid and piperine in formulation and found to be 0.049 % and 0.051 % respectively in formulation. In vitro antituberculosis activity was performed using Lowenstein Jensen Medium and percentage inhibition at 50 μg/ml, 500 μg/ml and 1000 μg/ml was found to be 11.71 %, 74.15 % and 94.11 % respectively. Results showed dose dependant in vitro antituberculosis activity. These activities may be due to the presence of various phytoconstituents like flavonoids, tannins and phenoilc in formulations. Above results also support the rational use of above plants as antituberculosis.