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Development and validation of HPTLC method for the estimation of clotrimazole in bulk drug and tablet formulation

Author(s): Parul Parmar, Ankita Mehta
L. M. College of Pharmacy, Navrangpura, Gujarat-380 009, India

Correspondence Address:
Parul Parmar K. B. Raval College of Pharmacy, Shertha, Gandhinagar-382 423 India

A simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed and validated for the determination of clotrimazole in bulk drug and tablet dosage form. The stationary phase used was precoated silica gel 60F 254 . The mobile phase used was a mixture of cyclohexane:toluene:methanol:triethyleamine (8:2:0.5:0.2 v/v/v/v). The detection of spot was carried out at 262 nm. The method was validated in terms of linearity, accuracy, precision and specificity. The calibration curve was found to be linear between 200 to 1000 ng/spot for clotrimazole. The limit of detection and the limit of quantification for clotrimazole were found to be 50 ng/spot and 200 ng/spot, respectively. The proposed method can be successfully used to determine the drug content of bulk drug and marketed formulation of tablet.

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