Abstract

To investigate the role of circ_0084927 in lung cancer cell sensitivity to cisplatin. Half-maximal inhibitory concentration value of cisplatin was detected by cell counting kit-8. circ_0084927 and microRNA-634 expression were determined by reverse transcription quantitative polymerase chain reaction. Cell counting kit-8, scratch healing, colony formation as well as Transwell experiments were used for analyzing cell viability, scratch healing rate, clone number and invasive ability, respectively. Dualluciferase reporter method was utilized to validate targeting relationship. The half-maximal inhibitory concentration value of cisplatin and circ_0084927 expression were increased in A549/DDP cells, whereas microRNA-634 expression displayed an opposite result in comparison with A549 cells (p<0.05). Relative to the DDP+si-NC group, the cell optical density value, scratch healing rate, clone numbers and invasion numbers were significantly reduced in the DDP+si-circ_0084927 group (p<0.05). Optical density value, scratch healing rate, clone numbers as well as invasion numbers in the DDP+microRNA-634 group were significantly reduced relative to the DDP+microRNA-NC group (p<0.05). Circ_0084927 directly bound to microRNA-634. Relative to the DDP+si-circ_0084927+anti-microRNA-NC group, optical density value, scratch healing rate, clone numbers as well as invasion numbers in the DDP+si-circ_0084927+antimicroRNA- 634 group were increased (p<0.05). Interference with circ_0084927 reduced the cisplatin resistance of lung cancer cells by promoting microRNA-634.