All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

Effect and Association of circ_0084927 and microRNA-634 on Lung Cancer Cell Sensitivity to Cisplatin

Author(s): Yang Gao, Rui Wang, Li Zhang, Li Wang, Xiaocui Huang and Xiaoyun Xu*
College of Education and Humanities, Suzhou Vocational University, Suzhou, Jiangsu Province 215104, 1College of Science, Liaoning Technical University, Fuxin, Liaoning Province 123008, 2Fuxin Industrial Technology Innovation and Promotion Center, Fuxin Industrial Technology Research Institute, Fuxin, Liaoning Province 123000, 3Department of General Surgery, The Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong Province 515051, China

Correspondence Address:
Xiaoyun Xu, Department of General Surgery, The Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong Province 515051, China, E-mail: meiyangyang1304@126.com


To investigate the role of circ_0084927 in lung cancer cell sensitivity to cisplatin. Half-maximal inhibitory concentration value of cisplatin was detected by cell counting kit-8. circ_0084927 and microRNA-634 expression were determined by reverse transcription quantitative polymerase chain reaction. Cell counting kit-8, scratch healing, colony formation as well as Transwell experiments were used for analyzing cell viability, scratch healing rate, clone number and invasive ability, respectively. Dualluciferase reporter method was utilized to validate targeting relationship. The half-maximal inhibitory concentration value of cisplatin and circ_0084927 expression were increased in A549/DDP cells, whereas microRNA-634 expression displayed an opposite result in comparison with A549 cells (p<0.05). Relative to the DDP+si-NC group, the cell optical density value, scratch healing rate, clone numbers and invasion numbers were significantly reduced in the DDP+si-circ_0084927 group (p<0.05). Optical density value, scratch healing rate, clone numbers as well as invasion numbers in the DDP+microRNA-634 group were significantly reduced relative to the DDP+microRNA-NC group (p<0.05). Circ_0084927 directly bound to microRNA-634. Relative to the DDP+si-circ_0084927+anti-microRNA-NC group, optical density value, scratch healing rate, clone numbers as well as invasion numbers in the DDP+si-circ_0084927+antimicroRNA- 634 group were increased (p<0.05). Interference with circ_0084927 reduced the cisplatin resistance of lung cancer cells by promoting microRNA-634.

Full-Text | PDF

 
 
Google scholar citation report
Citations : 69022

Indian Journal of Pharmaceutical Sciences received 69022 citations as per google scholar report