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Abstract

Effect of Artemisinin Combined with Allicin on Improving Cardiac Function, Fibrosis and Nuclear Factor-Kappa B Signaling Pathway in Rats with Diabetic Cardiomyopathy

Author(s): L. Kong*, Xiaoqing Ji, Yan Liu and Y. Du
Department of Cardiology, Chengde Medical College, Collage of Traditional Chinese Medicine, Chengde, Hebei 067050, China

Correspondence Address:
L. Kong, Department of Cardiology, Chengde Medical College, Collage of Traditional Chinese Medicine, Chengde, Hebei 067050, China, E-mail: [email protected]

This study aimed to see how artemisinin and allicin affected heart function, myocardial fibrosis and regulating the nuclear factor-B signaling pathways in the myocardial tissue of diabetic cardiomyopathy rats. 50 rats were selected, 10 of which were kept normally without any intervention as the rest 40 were in the normal group injected intraperitoneally 65 μg/g streptozotocin at one time to construct diabetic cardiomyopathy model. 37 rats meeting the criteria for successful model establishment were randomly divided into ten rodents in the model category, 9 rats each in the artemisinin, allicin and combination groups. For 4 w, the artemisinin group received 75 mg/kg of artemisinin, the allicin group received 40 mg/kg of allicin and the combination group received the same doses of artemisinin and allicin gavage as the artemisinin and allicin groups. The combined group had significantly lower expression of left ventricular end-diastolic internal diameter, left ventricular end-systolic internal diameter, left ventricular ejection fraction, fractional shortening, E/A, nuclear factor-B signaling pathway protein nuclear factor- B-p65 and p-nuclear factor-B-p65 than the artemisinin and allicin groups (p<0.05). For comparing the cardiac function indicators left ventricular end-diastolic internal diameter, left ventricular end-systolic internal diameter, left ventricular ejection fraction, fractional shortening, E/A, nuclear factor-B signaling pathway protein nuclear factor-B-p65 and p-nuclear factor-B-p65 expressions, there was no statistical difference here between artemisinin and allicin groups (p>0.05). After observing the myocardial fibrosis in each group, we found the collagen fibers-associated disorder arrangement of the proliferative network in the modeled group, formation of the fibrous scar with large volume, cardiac hypertrophy, inconsistent coloration, nucleus consolidation, disintegration and even removal. When compared to the model group, the artemisinin group, allicin group and combined group all demonstrated various degrees of improvement in the problematic structure with more intact muscle fibers, neater arrangement, more normal cell morphology and more homogeneous staining, with the most significant improvement in the combined group. Compared with artemisinin and allicin alone, artemisinin combined with allicin improved cardiac dysfunction and reduced myocardial fibrosis in rats with diabetic cardiomyopathy and both may act via promoting the inactivation of the nuclear factor-kappa B signaling cascade.

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Citations : 53647

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