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Abstract

Effect of Certain Quinones on Adenosine Triphosphate Level in Human Bladder Cancer Cells

Author(s): H. Zhu1 and H. Wang*
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, 1University of Chinese Academy of Sciences, Beijing 100049, China

Correspondence Address:
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China, E-mail: [email protected]

The aim of this study was to reveal the relationship between four kinds of quinones and the ATP level in human bladder cancer T24 cells. Quinones could cause damage to T24 cells, and after 12 h exposure, IC50 values were in μM, 74 for 2-chlorobenzoquinone, 82 for 2,5-dichloro-1,4-benzoquinone, 122 for 2,6-dichloro-1,4-benzoquinone and 45 for 2,3,6-trichloro-1,4-benzoquinone. Further, the mechanism of increased ATP level when exposed to the 4 quinones was studied. It could be due to increased reactive oxygen species level in T24 cells when exposed to the these quinones, which suppressed the ATPase activity, leading to increased levels of ATP, which was dose-dependent. The higher the quinone concentration was, the higher the reactive oxygen species level and the ATPase activity was lower, leading to accumulation of more ATP in T24 cells. This study indicated that these 4 quinones could block energy metabolism in T24 cells which could lead to cell death.

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