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Abstract

Effect of Diphosphate on Proliferation of Osteoblasts in Mice

Author(s): WENXIONG HU, J. JIANG, AND HUA LI*
Department of Orthopaedics, Hainan West Central Hospital, Danzhou City, Hainan Province-571700, China

Correspondence Address:
HUA LI, Department of Orthopaedics, Hainan West Central Hospital, Danzhou City, Hainan Province-571700, China, E-mail: [email protected]


In order to investigate the effect of diphosphate on the proliferation and differentiation of mouse osteoblasts and to further explore the optimal concentration of diphosphate, mouse embryo osteoblasts precursor cells are used as the research object. The expression level of its messenger ribonucleic acid is detected by real-time quantitative polymerase chain reaction (real-time polymerase chain reaction). 3- (4, 5) - dimethylahiazo (-z-yl) -3, 5-di-phenytetrazolium bromide method is used to detect the proliferation of mouse embryo osteoblasts precursor cells and its effect on alkaline phosphatase activity, so as to explore its correlation. The results show that mouse embryo osteoblasts precursor cells have the greatest value-added effect, the most significant gene expression and the highest alkaline phosphatase activity at the concentration of 10-8 M diphosphate. The results are as expected. More accurate, standardized and quantifiable data for mouse embryo osteoblasts precursor cell proliferation are obtained through experiments. There are still some shortcomings in the research process, but the results of this study still provide some guidance for exploring more precise mechanism of action in the future. Therefore, the results of this study provide guidance for exploring more precise mechanisms in the future, which is the value of this study.

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